Anti-S100A16 antibody (ab130419)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-S100A16 antibody
See all S100A16 primary antibodies -
Description
Rabbit polyclonal to S100A16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment: VIVLVENFYK YVSKYSLVKN KISKSSFREM LQKELNHMLS DTGNRKAADK LIQNLDANHD GRISFDEYWT LIGGITGPIA KLIHEQEQ, corresponding to amino acids 12-99 of Human S100A16 (UniProt: Q96FQ6).
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Positive control
- RT4, U251 MG, Human Liver and Human tonsil lysates; Human esophagus tissue; A431 cells.
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General notes
ab130149 is a mono-specific antibody.
The antibody solution should be gently mixed before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab130419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250 - 1/500. Predicted molecular weight: 11 kDa. | |
| IHC-P | 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization. |
Target
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Function
Calcium-binding protein. Binds one calcium ion per monomer. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Primarily nucleolar. Translocates from the nucleolus to the cytoplasm in response to an increase of intracellular calcium levels. - Information by UniProt
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Database links
- Entrez Gene: 140576 Human
- Entrez Gene: 361991 Rat
- SwissProt: Q96FQ6 Human
- Unigene: 515714 Human
- Unigene: 1053 Rat
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Alternative names
- 2300002L21Rik antibody
- AAG13 antibody
- Aging associated gene 13 protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (ab130419)ab130419, at a dilution of 1/1000, staining S100A16 in paraffin-embedded Human esophagus tissue by Immunohistochemistry. -
Western blot - Anti-S100A16 antibody (ab130419)All lanes : Anti-S100A16 antibody (ab130419) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 11 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (ab130419)ab130419, at a concentration of 4 µg/ml, staining S100A16 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Katono K et al. S100A16, a promising candidate as a prognostic marker for platinum-based adjuvant chemotherapy in resected lung adenocarcinoma. Onco Targets Ther 10:5273-5279 (2017). IHC-P ; Human . Read more (PubMed: 29138580) »
