Overview

  • Product name

  • Description

    Rabbit polyclonal to S100A16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment: VIVLVENFYK YVSKYSLVKN KISKSSFREM LQKELNHMLS DTGNRKAADK LIQNLDANHD GRISFDEYWT LIGGITGPIA KLIHEQEQ, corresponding to amino acids 12-99 of Human S100A16 (UniProt: Q96FQ6).

  • Positive control

    • RT4, U251 MG, Human Liver and Human tonsil lysates; Human esophagus tissue; A431 cells.
  • General notes

    ab130149 is a mono-specific antibody.
    The antibody solution should be gently mixed before use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab130419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 11 kDa.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • Function

    Calcium-binding protein. Binds one calcium ion per monomer.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Primarily nucleolar. Translocates from the nucleolus to the cytoplasm in response to an increase of intracellular calcium levels.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2300002L21Rik antibody
    • AAG13 antibody
    • Aging associated gene 13 protein antibody
    • Aging associated protein 13 antibody
    • Aging-associated gene 13 protein antibody
    • AI325039 antibody
    • AI663996 antibody
    • DT1P1A7 antibody
    • MGC17528 antibody
    • Protein S100 A16 antibody
    • Protein S100-A16 antibody
    • Protein S100-F antibody
    • Protein S100F antibody
    • S100 calcium binding protein A16 antibody
    • S100 calcium-binding protein A16 antibody
    • S100A16 antibody
    • S100F antibody
    • S10AG_HUMAN antibody
    see all

Images

  • ab130419, at a dilution of 1/1000, staining S100A16 in paraffin-embedded Human esophagus tissue by Immunohistochemistry.
  • All lanes : Anti-S100A16 antibody (ab130419) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Predicted band size: 11 kDa

  • ab130419, at a concentration of 4 µg/ml, staining S100A16 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

References

This product has been referenced in:

  • Katono K  et al. S100A16, a promising candidate as a prognostic marker for platinum-based adjuvant chemotherapy in resected lung adenocarcinoma. Onco Targets Ther 10:5273-5279 (2017). IHC-P ; Human . Read more (PubMed: 29138580) »
See 1 Publication for this product

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