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Anti-S100A16 antibody (ab130419)

  • Datasheet
Submit a review Submit a question References (4)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (ab130419)
  • Western blot - Anti-S100A16 antibody (ab130419)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (ab130419)

Key features and details

  • Rabbit polyclonal to S100A16
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-S100A16 antibody
    See all S100A16 primary antibodies
  • Description

    Rabbit polyclonal to S100A16
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment: VIVLVENFYK YVSKYSLVKN KISKSSFREM LQKELNHMLS DTGNRKAADK LIQNLDANHD GRISFDEYWT LIGGITGPIA KLIHEQEQ, corresponding to amino acids 12-99 of Human S100A16 (UniProt: Q96FQ6).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • RT4, U251 MG, Human Liver and Human tonsil lysates; Human esophagus tissue; A431 cells.
  • General notes

    ab130149 is a mono-specific antibody.
    The antibody solution should be gently mixed before use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human S100A16 protein (ab105607)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab130419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 11 kDa.
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 11 kDa.
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Target

  • Function

    Calcium-binding protein. Binds one calcium ion per monomer.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm. Primarily nucleolar. Translocates from the nucleolus to the cytoplasm in response to an increase of intracellular calcium levels.
  • Target information above from: UniProt accession Q96FQ6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 140576 Human
    • SwissProt: Q96FQ6 Human
    • Unigene: 515714 Human
    • Alternative names

      • 2300002L21Rik antibody
      • AAG13 antibody
      • Aging associated gene 13 protein antibody
      • Aging associated protein 13 antibody
      • Aging-associated gene 13 protein antibody
      • AI325039 antibody
      • AI663996 antibody
      • DT1P1A7 antibody
      • MGC17528 antibody
      • Protein S100 A16 antibody
      • Protein S100-A16 antibody
      • Protein S100-F antibody
      • Protein S100F antibody
      • S100 calcium binding protein A16 antibody
      • S100 calcium-binding protein A16 antibody
      • S100A16 antibody
      • S100F antibody
      • S10AG_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (ab130419)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (ab130419)
      ab130419, at a dilution of 1/1000, staining S100A16 in paraffin-embedded Human esophagus tissue by Immunohistochemistry.
    • Western blot - Anti-S100A16 antibody (ab130419)
      Western blot - Anti-S100A16 antibody (ab130419)
      All lanes : Anti-S100A16 antibody (ab130419) at 1/250 dilution

      Lane 1 : RT4 cell lysate
      Lane 2 : U251 MG cell lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Predicted band size: 11 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (ab130419)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (ab130419)
      ab130419, at a concentration of 4 µg/ml, staining S100A16 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab130419? Please let us know so that we can cite the reference in this datasheet.

    ab130419 has been referenced in 4 publications.

    • Wang C  et al. S100A16 regulated by Snail promotes the chemoresistance of nonmuscle invasive bladder cancer through the AKT/Bcl-2 pathway. Cancer Manag Res 11:2449-2456 (2019). PubMed: 31118765
    • Zhang R  et al. S100A16-induced adipogenesis is associated with up-regulation of 11 ß-hydroxysteroid dehydrogenase type 1 (11ß-HSD1). Biosci Rep 39:N/A (2019). PubMed: 31399502
    • Kan J  et al. S100A16, a novel lipogenesis promoting factor in livers of mice and hepatocytes in vitro. J Cell Physiol 234:21395-21406 (2019). PubMed: 31069793
    • Katono K  et al. S100A16, a promising candidate as a prognostic marker for platinum-based adjuvant chemotherapy in resected lung adenocarcinoma. Onco Targets Ther 10:5273-5279 (2017). IHC-P ; Human . PubMed: 29138580

    Customer reviews and Q&As

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