Anti-S100A16 antibody (ab130419)
Key features and details
- Rabbit polyclonal to S100A16
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-S100A16 antibody
See all S100A16 primary antibodies -
Description
Rabbit polyclonal to S100A16 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
Recombinant fragment: VIVLVENFYK YVSKYSLVKN KISKSSFREM LQKELNHMLS DTGNRKAADK LIQNLDANHD GRISFDEYWT LIGGITGPIA KLIHEQEQ, corresponding to amino acids 12-99 of Human S100A16 (UniProt: Q96FQ6).
-
Positive control
- RT4, U251 MG, Human Liver and Human tonsil lysates; Human esophagus tissue; A431 cells.
-
General notes
ab130149 is a mono-specific antibody.
The antibody solution should be gently mixed before use.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 11 kDa.
|
|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 11 kDa. |
|
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
-
Function
Calcium-binding protein. Binds one calcium ion per monomer. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Primarily nucleolar. Translocates from the nucleolus to the cytoplasm in response to an increase of intracellular calcium levels. - Information by UniProt
-
Database links
- Entrez Gene: 140576 Human
- SwissProt: Q96FQ6 Human
- Unigene: 515714 Human
-
Alternative names
- 2300002L21Rik antibody
- AAG13 antibody
- Aging associated gene 13 protein antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A16 antibody (ab130419)ab130419, at a dilution of 1/1000, staining S100A16 in paraffin-embedded Human esophagus tissue by Immunohistochemistry.
-
Western blot - Anti-S100A16 antibody (ab130419)All lanes : Anti-S100A16 antibody (ab130419) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 11 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-S100A16 antibody (ab130419)ab130419, at a concentration of 4 µg/ml, staining S100A16 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
Protocols
Datasheets and documents
References (4)
ab130419 has been referenced in 4 publications.
- Wang C et al. S100A16 regulated by Snail promotes the chemoresistance of nonmuscle invasive bladder cancer through the AKT/Bcl-2 pathway. Cancer Manag Res 11:2449-2456 (2019). PubMed: 31118765
- Zhang R et al. S100A16-induced adipogenesis is associated with up-regulation of 11 ß-hydroxysteroid dehydrogenase type 1 (11ß-HSD1). Biosci Rep 39:N/A (2019). PubMed: 31399502
- Kan J et al. S100A16, a novel lipogenesis promoting factor in livers of mice and hepatocytes in vitro. J Cell Physiol 234:21395-21406 (2019). PubMed: 31069793
- Katono K et al. S100A16, a promising candidate as a prognostic marker for platinum-based adjuvant chemotherapy in resected lung adenocarcinoma. Onco Targets Ther 10:5273-5279 (2017). IHC-P ; Human . PubMed: 29138580
