Product nameAnti-S100A3 antibody
See all S100A3 primary antibodies
DescriptionRabbit polyclonal to S100A3
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog
- Human skin tissue or OVCAR3 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab62277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 12 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/1562500.
FunctionBinds both calcium and zinc. Probably binds 2 zinc ions per molecule. May be involved in calcium-dependent cuticle cell differentiation and hair shaft formation.
Tissue specificitySkin specific.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Protein S 100E antibody
- Protein S-100E antibody
- Protein S100 A3 antibody
Anti-S100A3 antibody (ab62277) at 5 µg/ml + OVCAR3 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,000
Predicted band size: 12 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling S100A3 with ab62277 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.
ab62277 has not yet been referenced specifically in any publications.