Key features and details
- Goat polyclonal to S100A4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-S100A4 antibody
See all S100A4 primary antibodies
DescriptionGoat polyclonal to S100A4
SpecificityBoth variants (NP_002952.1; NP_062427.1) represent identical protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- Human Placenta lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab58597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
1 hour primary incubation is recommended for this product.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
Anti-S100A4 antibody (ab58597) at 0.03 µg/ml + Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 12 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab58597 at a concentration of 2µg/ml staining S100A4 in lymphatic cells in the lamina propria of human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Antigen retrieval was with a citrate buffer.
ab58597 has been referenced in 1 publication.
- Rasanen K et al. Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target. Mol Cell Proteomics 12:3778-92 (2013). PubMed: 24037664