Overview

  • Product name

  • Description

    Mouse monoclonal to S100A4
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.

  • Positive control

    • HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab93283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 (FSP1) antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 (MTS1) antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Images

  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 37.22 kDa
    why is the actual band size different from the predicted?



    Predicted band size for the immunogen in 37.22 kDa.

    This image was generated using the ascites version of the product.

  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa



    This image was generated using the ascites version of the product.

  • Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa



    This image was generated using the ascites version of the product.

  • All lanes : Anti-S100A4 antibody (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)

    Lane 1 : cell lysate from S100A4 transfected 293T cell line
    Lane 2 : cell lysate from non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 11.7 kDa why is the actual band size different from the predicted?



    This image was generated using the ascites version of the product.

  • ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.

    This image was generated using the ascites version of the product.

  • ab93283 detecting recombinant S100A4 (with a proprietary tag) by sandwich ELISA. The detection limit for recombinant S100A4 (with a proprietary tag) is approximately 0.1 ng/ml as a capture antibody.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Nguy?n-Thanh T  et al. Inhibition of histone deacetylase 1 ameliorates renal tubulointerstitial fibrosis via modulation of inflammation and extracellular matrix gene transcription in mice. Int J Mol Med 41:95-106 (2018). Read more (PubMed: 29115561) »
  • Zhang W  et al. S100a4 Is Secreted by Alternatively Activated Alveolar Macrophages and Promotes Activation of Lung Fibroblasts in Pulmonary Fibrosis. Front Immunol 9:1216 (2018). Read more (PubMed: 29910813) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated cell)
Specification
differentiated cell
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Nov 12 2012

Answer

Thank you for contacting us and your interest in our products.

I have looked into the three antibodies we have directed against S100A4 and whether they are likely to work when used in a sandwich ELISA. We have the following antibodies directed against S100A4 which have been used in ELISA previously:

Mouse monoclonal to S100A4 (ab93283)

Mouse monoclonal [NJ4F3] to S100A4 (ab68124)

Goat polyclonal to S100A4 (ab115699)

Of these, only ab93283 has been used with mouse samples previously. However, as the immunogens used to raise ab68124 and ab115699 shares high homology with the mouse protein we would expect them to cross react. As we have not tested it ourselves we would however not be able to guarantee this.

None of the antibodies have been used as a pair in sandwich ELISA before and we would therefore not be able to guarantee that they would work together. The two mouse monoclonal antibodies are raised against immunogens which cover most of the protein length, including the region used to produce the goat polyclonal. Theoretically, the antibodies could therefore bind the same region and not be compatible. However, as we have not tested it, we would not be able to say.

If you would like to test ab93283 and ab115699 as a pair in a sandwich ELISA you would be eligable for a testing discount. This offer means that if you purchaseab93283 and ab115699 as normal, test the antibodies with yourmouse samples in ELISA and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price ofab93283or ab115699 off any product). More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab93283 and ab115699.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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