Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)

Overview

  • Product name

    Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free
    See all S100A4 primary antibodies
  • Description

    Rabbit monoclonal [EPR14639(2)] to S100A4 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Based on sequence homologies, the antibody may cross-react with other proteins of the same family (S100A1-12). We did not perform any experiments to confirm this.

    We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: IP, Flow Cyt, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus.
    Database link: P26447

  • Positive control

    • WB: A549, A375, Human fetal spleen lysates. IHC-P: Human cervix carcinoma, lung carcinoma and gastric carcinoma tissues. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells.
  • General notes

    Ab220213 is the carrier-free version of ab197896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab220213 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab220213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

Target

  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 (FSP1) antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 (MTS1) antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used ay 1/500 dilution as a secondary antibody and cells were counterstained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Nuclear and cytoplasm staining on cervix carcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • ab197896 at 1/40 immunoprecipitating S100A4 in A549 whole cell lysate observed at 12 KDa.

    Lane 1 (input): A549 whole cell lysate 10μg

    Lane 2 (+): ab197896 + A549 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab197896 in A549 whole cell lysate

    For western blotting, Panel A: ab197896, 1:1000; Panel B: ab124805, 1:1000 and anti-rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

  • ab197896 at 1/40 immunoprecipitating S100A4 in A549 whole cell lysate observed at 12 KDa.

    Lane 1 (+): ab197896 + A549 whole cell lysate.

    Lane 2 (-): Rabbit monoclonal IgG (ab172730) instead of ab197896 in A549 whole cell lysate

    For western blotting, ab197896 at 1/1000 and anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/1500).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

  • Flow Cytometry analysis of Jurkat cells labelling S100A4 with ab197896 at 1/250 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at a dilution of 1/500 and cells were counterstained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Nuclear and weakly staining on lung carcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at 1/500 dilution. Cells were counterstained with Hematoxylin. 

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Cytoplasm and nuclear staining on human gastric carcinoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197896).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Liu Y  et al. Extracellular ATP drives breast cancer cell migration and metastasis via S100A4 production by cancer cells and fibroblasts. Cancer Lett 430:1-10 (2018). Read more (PubMed: 29733962) »
See 1 Publication for this product

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