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    s100a4-antibody-epr27612-ab124805.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-S100A4 antibody [EPR2761(2)] (ab124805)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (42)

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Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Flow Cytometry (Intracellular) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Flow Cytometry (Intracellular) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
  • Anti-S100A4 antibody [EPR2761(2)] (ab124805)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2761(2)] to S100A4
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-S100A4 antibody [EPR2761(2)]
    See all S100A4 primary antibodies
  • Description

    Rabbit monoclonal [EPR2761(2)] to S100A4
  • Host species

    Rabbit
  • Specificity

    Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for suitable positive controls.

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human S100A4 aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P26447
    (Peptide available as ab232852)

  • Positive control

    • Human tonsil, A549, A375, HeLa and Human small intestine lysates; Human tonsil tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2761(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Myocardial regeneration

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-S100A4 antibody [EPR2761(2)] (ab196168)
    • Alexa Fluor® 488 Anti-S100A4 antibody [EPR2761(2)] (ab196380)
    • Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human S100A4 knockout A549 cell line (ab261865)
    • Human S100A4 knockout HeLa cell line (ab265709)
  • KO cell lysates

    • Human S100A4 knockout HeLa cell lysate (ab257046)
    • Human S100A4 knockout A549 cell lysate (ab261674)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human S100A4 protein (ab85489)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
1/100 - 1/250.
Flow Cyt (Intra)
1/80 - 1/800.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB (1)
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IP
1/10 - 1/100.
IHC-P (4)
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/80 - 1/800.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IP
1/10 - 1/100.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Target information above from: UniProt accession P26447 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6275 Human
    • Omim: 114210 Human
    • SwissProt: P26447 Human
    • Unigene: 654444 Human
    • Alternative names

      • 18A2 antibody
      • 42A antibody
      • calcium Placental protein antibody
      • Calvasculin antibody
      • CAPL antibody
      • Fibroblast specific protein 1 (FSP1) antibody
      • Fibroblast specific protein 1 antibody
      • Fibroblast specific protein antibody
      • FSP1 antibody
      • Leukemia multidrug resistance associated protein antibody
      • Malignant transformation suppression 1 (MTS1) antibody
      • Malignant transformation suppression 1 antibody
      • Metastasin antibody
      • MTS1 antibody
      • OTTHUMP00000015467 antibody
      • OTTHUMP00000015468 antibody
      • P9KA antibody
      • PEL98 antibody
      • Placental calcium-binding protein antibody
      • Protein Mts1 antibody
      • Protein S100 A4 antibody
      • Protein S100-A4 antibody
      • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
      • S100 calcium binding protein A4 antibody
      • S100 calcium-binding protein A4 antibody
      • S100a4 antibody
      • S10A4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      All lanes : Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : S100A4 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 12 kDa
      Observed band size: 11 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab124805 observed at 11 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

      ab124805 was shown to react with S100A4 in wild-type HeLa cells in Western blot with loss of signal observed in S100A4 knockout cell line ab265709 (S100A4 knockout cell lysate ab257046). Wild-type HeLa and S100A4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab124805 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      All lanes : Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1/1000 dilution

      Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 2 : S100A4 knockout A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 3 : A-375 (Human malignant melanoma cell line) whole cell lysate
      Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 12 kDa
      Observed band size: 12 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab124805 observed at 12 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

      ab124805 was shown to react with S100A4 in wild-type A549 cells in Western blot with loss of signal observed in S100A4 knockout cell line ab261865 (knockout cell lysate ab261674). Wild-type A549 and S100A4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab124805 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] (ab124805)This image is courtesy of an anonymous Abreview

      Unpurified ab124805 staining S100A4 in the A549 cell line from Human lungs by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for 45 minutes at 25°C. A TRITC-conjugated Goat anti-rabbit  polyclonal was used as the secondary antibody.

      See Abreview

    • Flow Cytometry (Intracellular) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Flow Cytometry (Intracellular) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)

      ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 80% methanoland permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/800. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: ab172730 rabbit monoclonal IgG (Black) 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

       

       

    • Flow Cytometry (Intracellular) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Flow Cytometry (Intracellular) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)

      ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 80% methanoland permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: ab172730 rabbit monoclonal IgG (Black) 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)

      ab124805 staining S100A4 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      All lanes : Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1/1000 dilution (unpurified)

      Lane 1 : Human tonsil lysate
      Lane 2 : A549 cell lysate
      Lane 3 : A375 cell lysate
      Lane 4 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 12 kDa
      Observed band size: 12 kDa

    • Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] (ab124805)

      ab124805 immunoprecipitating S100A4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a dilution of 1/1500.

      Lane 1: Human tonsil whole cell lysate (10ug)
      Lane 2: Human tonsil whole cell lysate 
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124805 in human tonsil whole cell lysate

    • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Anti-S100A4 antibody [EPR2761(2)] (ab124805) + A549 (human lung carcinoma) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 12 kDa
      Additional bands at: 12 kDa. We are unsure as to the identity of these extra bands.



      Blocking buffer: 5% NFDM /TBST 

      Diluting Buffer: 5% NFDM /TBST 

    • Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Western blot - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      All lanes : Anti-S100A4 antibody [EPR2761(2)] (ab124805) at 1/2500 dilution

      Lane 1 : Human tonsil tissue lysate
      Lane 2 : A375 (human malignant melanoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 12 kDa
      Additional bands at: 12 kDa. We are unsure as to the identity of these extra bands.



      Blocking Buffer: 5% NFDM /TBST

      Diluting Buffer: 5% NFDM /TBST 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)

      Unpurified ab124805, at 1/250 dilution, staining S100A4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] (ab124805)This image is courtesy of an anonymous Abreview

      Immunohistochemical analysis of Human lung tissue, staining S100A4 with unpurified ab124805.

      Tissue was fixed with HOPE and blocked with blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] (ab124805)

      ab124805 staining S100A4 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/500. ab7291 and ab150120 were used as counterstains for primary antibody ab124805 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    • Anti-S100A4 antibody [EPR2761(2)] (ab124805)
      Anti-S100A4 antibody [EPR2761(2)] (ab124805)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (42)

    Publishing research using ab124805? Please let us know so that we can cite the reference in this datasheet.

    ab124805 has been referenced in 42 publications.

    • Cao X  et al. MSC-derived exosomal lncRNA SNHG7 suppresses endothelial-mesenchymal transition and tube formation in diabetic retinopathy via miR-34a-5p/XBP1 axis. Life Sci 272:119232 (2021). PubMed: 33600866
    • Singh M  et al. 22(R)-hydroxycholesterol for dopaminergic neuronal specification of MSCs and amelioration of Parkinsonian symptoms in rats. Cell Death Discov 7:13 (2021). PubMed: 33454721
    • Lyu T  et al. Exosomes from BM-MSCs promote acute myeloid leukemia cell proliferation, invasion and chemoresistance via upregulation of S100A4. Exp Hematol Oncol 10:24 (2021). PubMed: 33789743
    • Zhang HW  et al. Cancer-associated fibroblast-derived exosomal microRNA-24-3p enhances colon cancer cell resistance to MTX by down-regulating CDX2/HEPH axis. J Cell Mol Med 25:3699-3713 (2021). PubMed: 33621425
    • Mussa BM  et al. Differentiated PDGFRa-Positive Cells: A Novel In-Vitro Model for Functional Studies of Neuronal Nitric Oxide Synthase. Int J Mol Sci 22:N/A (2021). PubMed: 33805311
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-8 of 8 Abreviews

    Western blot abreview for Anti-S100A4 antibody [EPR2761(2)]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    10 µg
    Specification
    smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Luis Miguel Cardoso Dos Santos

    Verified customer

    Submitted Feb 24 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A4 antibody [EPR2761(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    Leica IHC Super blocking as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 01 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-S100A4 antibody [EPR2761(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rhesus monkey Cell (Dermal Fibroblast)
    Permeabilization
    Yes - 0.02% Triton X-100 with 0.1mg/mL BSA in PBS, 7 min
    Specification
    Dermal Fibroblast
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 23 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A4 antibody [EPR2761(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
    Permeabilization
    Yes - 0.25%Triton X100 in PBS
    Specification
    skin
    Blocking step
    Dako X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Hongwei Shao

    Verified customer

    Submitted Sep 29 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A4 antibody [EPR2761(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 06 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-S100A4 antibody [EPR2761(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Casein as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 23°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 26 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-S100A4 antibody [EPR2761(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Specification
    A549
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton X-100/PBS
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A4 antibody [EPR2761(2)]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung tissue)
    Specification
    Lung tissue
    Fixative
    HOPE
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Zytomed Systems HRP Polymer Block Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 11 2013

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