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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
  • Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2761(2)] to S100A4 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free
    See all S100A4 primary antibodies
  • Description

    Rabbit monoclonal [EPR2761(2)] to S100A4 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for suitable positive controls.

  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Positive control

    • Human tonsil, A549, A375 and Human small intestine lysates; Human tonsil tissue
  • General notes

    ab216003 is the carrier-free version of ab124805 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab216003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2761(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Myocardial regeneration

Associated products

  • Alternative Versions

    • Anti-S100A4 antibody [EPR2761(2)] (ab124805)
    • Alexa Fluor® 647 Anti-S100A4 antibody [EPR2761(2)] (ab196168)
    • Alexa Fluor® 488 Anti-S100A4 antibody [EPR2761(2)] (ab196380)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human S100A4 protein (ab85489)

Applications

Our Abpromise guarantee covers the use of ab216003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Target information above from: UniProt accession P26447 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6275 Human
    • Omim: 114210 Human
    • SwissProt: P26447 Human
    • Unigene: 654444 Human
    • Alternative names

      • 18A2 antibody
      • 42A antibody
      • calcium Placental protein antibody
      • Calvasculin antibody
      • CAPL antibody
      • Fibroblast specific protein 1 (FSP1) antibody
      • Fibroblast specific protein 1 antibody
      • Fibroblast specific protein antibody
      • FSP1 antibody
      • Leukemia multidrug resistance associated protein antibody
      • Malignant transformation suppression 1 (MTS1) antibody
      • Malignant transformation suppression 1 antibody
      • Metastasin antibody
      • MTS1 antibody
      • OTTHUMP00000015467 antibody
      • OTTHUMP00000015468 antibody
      • P9KA antibody
      • PEL98 antibody
      • Placental calcium-binding protein antibody
      • Protein Mts1 antibody
      • Protein S100 A4 antibody
      • Protein S100-A4 antibody
      • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
      • S100 calcium binding protein A4 antibody
      • S100 calcium-binding protein A4 antibody
      • S100a4 antibody
      • S10A4_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

      ab124805 staining S100A4 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    • Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

      ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 80% methanol and permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/800. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: ab172730 rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    • Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

      ab124805 staining S100A4 in HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 80% methanol and permeabilised with 0.1% Triton X-100 (in PBS). The sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: ab172730 rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    • Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Immunoprecipitation - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

      ab124805 immunoprecipitating S100A4. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a dilution of 1/1500.

      Lane 1: Human tonsil whole cell lysate (10ug)
      Lane 2: Human tonsil whole cell lysate 
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124805 in human tonsil whole cell lysate

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

      ab124805 staining S100A4 in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/500. ab7291 and ab150120 were used as counterstains for primary antibody ab124805 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)This image is courtesy of an anonymous Abreview.

      Unpurified ab124805 staining S100A4 in the A549 cell line from Human lungs by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for 45 minutes at 25°C. A TRITC-conjugated Goat anti-rabbit  polyclonal was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

      Unpurified ab124805, at 1/250 dilution, staining S100A4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)This image is courtesy of an anonymous Abreview.

      Immunohistochemical analysis of Human lung tissue, staining S100A4 with unpurified ab124805.

      Tissue was fixed with HOPE and blocked with blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124805).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)
      Anti-S100A4 antibody [EPR2761(2)] - BSA and Azide free (ab216003)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab216003? Please let us know so that we can cite the reference in this datasheet.

    ab216003 has not yet been referenced specifically in any publications.

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