Anti-S100A4 antibody [S100A4/1481] (ab218511)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-S100A4 antibody [S100A4/1481]
See all S100A4 primary antibodies -
Description
Mouse monoclonal [S100A4/1481] to S100A4 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human S100A4.
Database link: P26447 -
Positive control
- IHC-P: Human placenta tissue. WB: HeLa, A549 and A375 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S100A4/1481 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab218511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Protein Array | Use at an assay dependent concentration. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa. | |
| IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 6275 Human
- Omim: 114210 Human
- SwissProt: P26447 Human
- Unigene: 654444 Human
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Alternative names
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
Images
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![Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-345770-anti-s100a4-antibody-s100a41481-westernblot-human.jpg)
Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/ml
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : S100A4 knockout A549 whole cell lysate
Lane 3 : A375 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 12 kDa
Observed band size: 12 kDaLanes 1 - 4: Merged signal (red and green). Green - ab218511 observed at 12 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab218511 was shown to specifically react with S100A4 in wild-type A549 cells as signal was lost in S100A4 knockout cells. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. Ab218511 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Protein Array - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-336517-anti-s100a4-antibody-s100a4-1481-protein-array.jpg)
Protein Array - Anti-S100A4 antibody [S100A4/1481] (ab218511)ab218511 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-274179-anti-s100a4-antibody-s100a4-1481-western-blot.jpg)
Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A375 cell lysate
Predicted band size: 12 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-274178-anti-s100a4-antibody-s100a4-1481-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1481] (ab218511)Immunohistochemical analysis of formalin-fixed paraffin-embedded human placenta tissue, labeling S100A4 using ab218511 at 1 µg/mL.
Datasheets and documents
References
ab218511 has not yet been referenced specifically in any publications.
