Validated using a knockout cell line

Anti-S100A4 antibody [S100A4/1481] (ab218511)

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Key features and details

  • Mouse monoclonal [S100A4/1481] to S100A4
  • Suitable for: Protein Array, WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-S100A4 antibody [S100A4/1481]
    See all S100A4 primary antibodies

  • Description

    Mouse monoclonal [S100A4/1481] to S100A4

  • Host species

    Mouse

  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human S100A4.
    Database link: P26447

  • Positive control

    • IHC-P: Human placenta tissue. WB: HeLa, A549 and A375 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab218511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt

  • Database links

    • Alternative names

      • 18A2 antibody
      • 42A antibody
      • calcium Placental protein antibody
      • Calvasculin antibody
      • CAPL antibody
      • Fibroblast specific protein 1 (FSP1) antibody
      • Fibroblast specific protein 1 antibody
      • Fibroblast specific protein antibody
      • FSP1 antibody
      • Leukemia multidrug resistance associated protein antibody
      • Malignant transformation suppression 1 (MTS1) antibody
      • Malignant transformation suppression 1 antibody
      • Metastasin antibody
      • MTS1 antibody
      • OTTHUMP00000015467 antibody
      • OTTHUMP00000015468 antibody
      • P9KA antibody
      • PEL98 antibody
      • Placental calcium-binding protein antibody
      • Protein Mts1 antibody
      • Protein S100 A4 antibody
      • Protein S100-A4 antibody
      • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
      • S100 calcium binding protein A4 antibody
      • S100 calcium-binding protein A4 antibody
      • S100a4 antibody
      • S10A4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : S100A4 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 12 kDa
      Observed band size: 12 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab218511 observed at 12 kDa. Red - loading control ab181602 observed at 37 kDa.

       ab218511 Anti-S100A4 antibody [S100A4/1481] was shown to specifically react with S100A4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261758 (knockout cell lysate ab257045) was used. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. ab218511 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/ml

      Lane 1 : Wild-type A549 whole cell lysate
      Lane 2 : S100A4 knockout A549 whole cell lysate
      Lane 3 : A375 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 12 kDa
      Observed band size: 12 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab218511 observed at 12 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab218511 was shown to specifically react with S100A4 in wild-type A549 cells as signal was lost in S100A4 knockout cells. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. Ab218511 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Protein Array - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      Protein Array - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      ab218511 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : A375 cell lysate

      Predicted band size: 12 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1481] (ab218511)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1481] (ab218511)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded human placenta tissue, labeling S100A4 using ab218511 at 1 µg/mL.

    References (0)

    Publishing research using ab218511? Please let us know so that we can cite the reference in this datasheet.

    ab218511 has not yet been referenced specifically in any publications.

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