Key features and details
- Mouse monoclonal [S100A4/1482] to S100A4 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-S100A4 antibody [S100A4/1482] - BSA and Azide free
See all S100A4 primary antibodies
DescriptionMouse monoclonal [S100A4/1482] to S100A4 - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human S100A4. Exact sequence is proprietary.
Database link: P26447
- Human placenta tissue; A549, HeLa and A375 cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Purification notesab218954 was purified from Bioreactor Concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab218954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 min at RT).
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling S100A4 with ab218954 at 1 µg/ml.
All lanes : Anti-S100A4 antibody [S100A4/1482] - BSA and Azide free (ab218954) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A375 cell lysate
Predicted band size: 12 kDa
ab218954 has not yet been referenced specifically in any publications.