Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (FITC) (ab7429)

Overview

  • Product name
    Anti-S100A9 + Calprotectin (S100A8/A9 complex) antibody [MAC387] (FITC)
    See all S100A9 + Calprotectin (S100A8/A9 complex) primary antibodies
  • Description
    Mouse monoclonal [MAC387] to S100A9 + Calprotectin (S100A8/A9 complex) (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody recognises the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig, Monkey, Baboon, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Human monocytes

  • General notes

    Fluorescein isothiocyanate (FITC) isomer 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab7429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Membrane permeabilisation is required for this application.

 

 

 

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Images

  • ab7429 staining S100A9 + Calprotectin (FITC) in human peripheral blood granulocyte by Flow Cytometry analysis.

References

This product has been referenced in:
  • Monteiro LN  et al. Tumour-associated macrophages: Relation with progression and invasiveness, and assessment of M1/M2 macrophages in canine mammary tumours. Vet J 234:119-125 (2018). IHC-P . Read more (PubMed: 29680383) »
  • Burwitz BJ  et al. Technical advance: liposomal alendronate depletes monocytes and macrophages in the nonhuman primate model of human disease. J Leukoc Biol 96:491-501 (2014). Read more (PubMed: 24823811) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

This antibody recognized the L1 or Calprotectin molecule which is expressed by granulocytes, monocytes and tissue macrophages. In staining samples by flow cytometry which contains all of these relevant populations...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (macrophages (peritoneal))
Specification
macrophages (peritoneal)
Username

Mr. olivier Reynard

Verified customer

Submitted Jul 24 2006

After a careful search with the sources of our macrophage antibodies I regret to inform you that we have no products in our product range which can identify macrophage form monocyte by Western blot and are cross reactive with human. In our product ...

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This antibody has not yet been tested in frozen sections.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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