Key features and details
- Rat monoclonal [2B10] to S100A9
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-S100A9 antibody [2B10]
See all S100A9 primary antibodies
DescriptionRat monoclonal [2B10] to S100A9
SpecificitySpecific for mouse S100-A9 protein that forms a heterodimer with S100-A8 and constitutes 45% of the neutrophil cytosolic protein. Stains neutrophils strongly and monocytes weakly.
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse
Recombinant full length protein, corresponding to amino acids 1-113 of Mouse S100A9
- Neutrophils FFPE mouse lung tissue sections RAW246.7 cell line
General notesGrowth Conditions: RPMI 2% + 10% FCS and weaned down to 5% FCS
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab105472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
- Information by UniProt
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IHC image of S100A9 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105472, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab105472 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105472, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab98386, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab105472 has been referenced in 16 publications.
- Manils J et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. Elife 9:N/A (2020). PubMed: 32597759
- Hemshekhar M et al. Cathelicidin and Calprotectin Are Disparately Altered in Murine Models of Inflammatory Arthritis and Airway Inflammation. Front Immunol 11:1932 (2020). PubMed: 32973796
- Sullivan DP et al. Endothelial IQGAP1 regulates leukocyte transmigration by directing the LBRC to the site of diapedesis. J Exp Med 216:2582-2601 (2019). PubMed: 31395618
- Tan Y et al. Streamlining volumetric multi-channel image cytometry using hue-saturation-brightness-based surface creation. Commun Biol 1:136 (2018). PubMed: 30272015
- Casanova-Acebes M et al. Neutrophils instruct homeostatic and pathological states in naive tissues. J Exp Med 215:2778-2795 (2018). PubMed: 30282719