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    s100a9-antibody-2b10-ab105472.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-S100A9 antibody [2B10] (ab105472)

  • Datasheet
Reviews (1)Q&A (1)References (16)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [2B10] (ab105472)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [2B10] (ab105472)

Key features and details

  • Rat monoclonal [2B10] to S100A9
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-S100A9 antibody [2B10]
    See all S100A9 primary antibodies
  • Description

    Rat monoclonal [2B10] to S100A9
  • Host species

    Rat
  • Specificity

    Specific for mouse S100-A9 protein that forms a heterodimer with S100-A8 and constitutes 45% of the neutrophil cytosolic protein. Stains neutrophils strongly and monocytes weakly.
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-113 of Mouse S100A9

  • Positive control

    • Neutrophils FFPE mouse lung tissue sections RAW246.7 cell line
  • General notes

    Growth Conditions: RPMI 2% + 10% FCS and weaned down to 5% FCS

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    2B10
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Recombinant Protein

    • Recombinant Human S100A9 protein (ab95909)

Applications

Our Abpromise guarantee covers the use of ab105472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • Tissue specificity

    Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • Cellular localization

    Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Target information above from: UniProt accession P06702 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 20202 Mouse
    • SwissProt: P31725 Mouse
    • Unigene: 2128 Mouse
    • Alternative names

      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [2B10] (ab105472)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody [2B10] (ab105472)
      IHC image of S100A9 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab105472, 10µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [2B10] (ab105472)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A9 antibody [2B10] (ab105472)
      ICC/IF image of ab105472 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105472, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab98386, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (16)

    Publishing research using ab105472? Please let us know so that we can cite the reference in this datasheet.

    ab105472 has been referenced in 16 publications.

    • Manils J  et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. Elife 9:N/A (2020). PubMed: 32597759
    • Hemshekhar M  et al. Cathelicidin and Calprotectin Are Disparately Altered in Murine Models of Inflammatory Arthritis and Airway Inflammation. Front Immunol 11:1932 (2020). PubMed: 32973796
    • Sullivan DP  et al. Endothelial IQGAP1 regulates leukocyte transmigration by directing the LBRC to the site of diapedesis. J Exp Med 216:2582-2601 (2019). PubMed: 31395618
    • Tan Y  et al. Streamlining volumetric multi-channel image cytometry using hue-saturation-brightness-based surface creation. Commun Biol 1:136 (2018). PubMed: 30272015
    • Casanova-Acebes M  et al. Neutrophils instruct homeostatic and pathological states in naive tissues. J Exp Med 215:2778-2795 (2018). PubMed: 30282719
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A9 antibody [2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
    Permeabilization
    No
    Specification
    mouse spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 23°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2017

    Question

    I have a customer who needs to know the molecular mass of Anti-S100A9 that recognizes.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 14 2015

    Answer

    I am happy to inform you that the predicted molecular mass of S100A9 is around 13 kDa. You can find the expected molecular mass and other valuable information about this protein on UniProt (http://www.uniprot.org/uniprot/P31725). Please note that S100A9 forms a heterodimer with S100A8. In case your customer is planning to run a native gel expect a band of higher molecular weight.

    Read More

    Abcam Scientific Support

    Answered on Jul 14 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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