Key features and details
- Mouse monoclonal [47-8D3] to S100A9
- Suitable for: IHC-Fr, IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-S100A9 antibody [47-8D3]
See all S100A9 primary antibodies
DescriptionMouse monoclonal [47-8D3] to S100A9
Tested applicationsSuitable for: IHC-Fr, IHC-P, ELISA, WBmore details
Species reactivityReacts with: Human
Tissue/ cell preparation of peripheral blood monocyte components (Human).
- Lysate of human monocytes or granulocytes.
Can be used for phenotypical characterization of macrophages and myelomonocytic cells in situ.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Primary antibody notesCan be used for phenotypical characterization of macrophages and myelomonocytic cells in situ.
Our Abpromise guarantee covers the use of ab24111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 14 kDa.|
FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
- Information by UniProt
- Leukocyte L1 complex heavy chain antibody
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ab24111 has been referenced in 7 publications.
- Sill M et al. Protein Profiling Gastric Cancer and Neighboring Control Tissues Using High-Content Antibody Microarrays. Microarrays (Basel) 5:N/A (2016). IHC . PubMed: 27600085
- Sun W et al. Quantitative Proteomics Analysis of Tissue Interstitial Fluid for Identification of Novel Serum Candidate Diagnostic Marker for Hepatocellular Carcinoma. Sci Rep 6:26499 (2016). WB . PubMed: 27216119
- Gschwandtner M et al. Fetal Human Keratinocytes Produce Large Amounts of Antimicrobial Peptides: Involvement of Histone-Methylation Processes. J Invest Dermatol N/A:N/A (2014). PubMed: 24694903
- Fornander L et al. Airway symptoms and biological markers in nasal lavage fluid in subjects exposed to metalworking fluids. PLoS One 8:e83089 (2013). WB . PubMed: 24391738
- Gromov P et al. Up-regulated proteins in the fluid bathing the tumour cell microenvironment as potential serological markers for early detection of cancer of the breast. Mol Oncol 4:65-89 (2010). IHC-P ; Human . PubMed: 20005186
- Abtin A et al. The antimicrobial heterodimer S100A8/S100A9 (calprotectin) is upregulated by bacterial flagellin in human epidermal keratinocytes. J Invest Dermatol 130:2423-30 (2010). PubMed: 20555353
- Dowling P et al. Analysis of the saliva proteome from patients with head and neck squamous cell carcinoma reveals differences in abundance levels of proteins associated with tumour progression and metastasis. J Proteomics 71:168-75 (2008). PubMed: 18617144