Key features and details
- Rabbit polyclonal to S100A9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-S100A9 antibody
See all S100A9 primary antibodies
DescriptionRabbit polyclonal to S100A9
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Gorilla
- WB: Human spleen tissue and 293T cells transfected with S100A9.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab167576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa.|
FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
- Information by UniProt
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
Anti-S100A9 antibody (ab167576) at 1 µg/ml + Human spleen tissue at 50 µg
Predicted band size: 13 kDa
All lanes : Anti-S100A9 antibody (ab167576) at 1 µg/ml
Lane 1 : Lysate of 293T transfected with S100A9
Lane 2 : Lysate of untransfected 293T
Lysates/proteins at 15 µl per lane.
Predicted band size: 13 kDa
ab167576 has not yet been referenced specifically in any publications.