Anti-S100A9 antibody (ab167576)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-S100A9 antibody
See all S100A9 primary antibodies -
DescriptionRabbit polyclonal to S100A9
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Gorilla -
Immunogen
Full length protein corresponding to amino acids 1-114 of Human S100A9 (NP_002956.1).
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Positive control
- WB: Human spleen tissue and 293T cells transfected with S100A9.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 100% PBS -
Concentration information loading...
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PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab167576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa. |
Target
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FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
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Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
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Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
- Information by UniProt
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Database links
- Entrez Gene: 532569 Cow
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P28783 Cow
- SwissProt: P06702 Human
- Unigene: 112405 Human
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Alternative names
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
see all
Images
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Anti-S100A9 antibody (ab167576) at 1 µg/ml + Human spleen tissue at 50 µg
Predicted band size: 13 kDa -
All lanes : Anti-S100A9 antibody (ab167576) at 1 µg/ml
Lane 1 : Lysate of 293T transfected with S100A9
Lane 2 : Lysate of untransfected 293T
Lysates/proteins at 15 µl per lane.
Predicted band size: 13 kDa
Protocols
Datasheets and documents
References
ab167576 has not yet been referenced specifically in any publications.