Anti-S100A9 antibody (ab167576)
Key features and details
- Rabbit polyclonal to S100A9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S100A9 antibody
See all S100A9 primary antibodies -
Description
Rabbit polyclonal to S100A9 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Gorilla
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Immunogen
Full length protein corresponding to amino acids 1-114 of Human S100A9 (NP_002956.1).
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Positive control
- WB: Human spleen tissue and 293T cells transfected with S100A9.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab167576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa. |
Target
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Function
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
Tissue specificity
Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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Database links
- Entrez Gene: 532569 Cow
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P28783 Cow
- SwissProt: P06702 Human
- Unigene: 112405 Human
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Alternative names
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
see all
Images
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Western blot - Anti-S100A9 antibody (ab167576)Anti-S100A9 antibody (ab167576) at 1 µg/ml + Human spleen tissue at 50 µg
Predicted band size: 13 kDa -
Western blot - Anti-S100A9 antibody (ab167576)All lanes : Anti-S100A9 antibody (ab167576) at 1 µg/ml
Lane 1 : Lysate of 293T transfected with S100A9
Lane 2 : Lysate of untransfected 293T
Lysates/proteins at 15 µl per lane.
Predicted band size: 13 kDa
Datasheets and documents
References (0)
ab167576 has not yet been referenced specifically in any publications.
