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RecombinantRabMAb

Recombinant Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (ab251572)

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  • SDS
  • Certificate of Compliance
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Sandwich ELISA - Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (ab251572)
  • Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (ab251572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20066-233] to S100A9 - BSA and Azide free
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

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Recombinant Human S100A9 protein (ab95909)

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Overview

  • Product name

    Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free
    See all S100A9 primary antibodies
  • Description

    Rabbit monoclonal [EPR20066-233] to S100A9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    sELISA
    Recombinant fragment
    See all applications and species data
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    Ab251572 is the carrier-free version of ab222589. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251572 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20066-233
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Alternative Versions

    • Anti-S100A9 antibody [EPR20066-233] (ab222589)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Recombinant Protein

    • Recombinant Human S100A9 protein (ab95909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
sELISA
Recombinant fragment
Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration.
Notes
Sandwich ELISA
Use at an assay dependent concentration.

Target

  • Function

    Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • Tissue specificity

    Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
  • Cellular localization

    Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Target information above from: UniProt accession P06702 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6280 Human
    • Omim: 123886 Human
    • SwissProt: P06702 Human
    • Unigene: 112405 Human
    • Alternative names

      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Images

    • Sandwich ELISA - Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (ab251572)
      Sandwich ELISA - Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (ab251572)

      Standard Curve for S100A9 (Analyte: Recombinant human S100A9 protein) dilution range 0 ng/mL to 20 ng/mL using Capture antibody at 0.2ug/mL and Detector Antibody at 0.5ug/mL. Secondary antibody: Peroxidase Streptavidin SA-HRP at 1/20000 dilution. Concentration of ab222589 may vary from lot to lot; please use this curve as guideline.

      Washing buffer: 1X PBST
      Diluting/Blocking buffer and concentration: 1% BSA/PBS

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222589).

    • Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (ab251572)
      Anti-S100A9 antibody [EPR20066-233] - BSA and Azide free (ab251572)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab251572? Please let us know so that we can cite the reference in this datasheet.

    ab251572 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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