Recombinant
RabMAb

Recombinant Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free (ab243108)

Overview

  • Product name
    Anti-S100A9 antibody [EPR22332-75] - BSA and Azide free
    See all S100A9 primary antibodies
  • Description
    Rabbit monoclonal [EPR22332-75] to S100A9 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: IHC-Fr or IP
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse S100A9 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P31725

  • Positive control
    • IHC-P: Mouse spleen tissue; Rat spleen tissue. ICC/IF: Mouse spleen cells. Flow cyt: Mouse primary splenocytes.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab243108 is a PBS-only buffer format of ab242945. Please refer to ab242945 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab243108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IHC-Fr or IP.
  • Target

    • Function
      Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • Tissue specificity
      Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • Sequence similarities
      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • Cellular localization
      Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • Database links
    • Alternative names
      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling S100A9 with ab242945 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on granulocytes of rat spleen (PMID: 25860480) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).
    • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling S100A9 with ab242945 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on granulocytes of mouse spleen (PMID: 25860480) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).
    • Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary splenocytes labeling S100A9 with ab242945 at 1/600 dilution (right panel) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse spleen cells labeling S100A9 with ab242945 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of spleen cells (PMID: 2666142) is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242945).

    References

    ab243108 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab243108.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up