Overview

  • Product name

    Anti-S100A9 antibody [EPR3555]
    See all S100A9 primary antibodies
  • Description

    Rabbit monoclonal [EPR3555] to S100A9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human S100A9 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Breast cancer, Human tonsil, Human spleen and peripheral blood lymphocytes lysates, Human breast carcinoma tissue and Human spleen tissue. IF: DU145 cell line FC: Jurkat cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 13 kDa.
IHC-P 1/250 - 1/500.
ICC/IF 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
Flow Cyt 1/200.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • Tissue specificity

      Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • Cellular localization

      Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • Database links

    • Alternative names

      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Images

    • Anti-S100A9 antibody [EPR3555] (ab92507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling S100A9 with ab92507 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
      This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

    • ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
    • All lanes : Anti-S100A9 antibody [EPR3555] (ab92507) at 1/1000 dilution

      Lane 1 : Breast cancer lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Peripheral blood lymphocytes lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 13 kDa
      Observed band size: 14 kDa
      why is the actual band size different from the predicted?

    • Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling S100A9 (red) with purified ab92507 at a 1/200 dilution (10ug/mL). Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    • ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.
    • ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92507, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:

    • Wu DM  et al. S100A9 gene silencing inhibits the release of pro-inflammatory cytokines by blocking the IL-17 signalling pathway in mice with acute pancreatitis. J Cell Mol Med 22:2378-2389 (2018). IHC-P ; Mouse . Read more (PubMed: 29441717) »
    • Yang J  et al. Calcium-Binding Proteins S100A8 and S100A9: Investigation of Their Immune Regulatory Effect in Myeloid Cells. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29933628) »
    See all 13 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    As both antibodies contain Glycerol, they should be quite safe from Frost/Thaw damage (as they shouldn't freeze at all). In the case of these two antibodies the aliquots are rather a protection against contamination of the whole vial.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Answer

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any MRP8 and MRP14 antibody tested in the species and application you are using should be suitable for your applications.

    Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

    For your experiment, maybe an antibody that will bind to both proteins will be suitable? ab17050 recognises an epitope on the MRP8 + MRP14 complex which is not exposed on the individual subunits.

    https://www.abcam.com/index.html?datasheet=17050 (or use the following: https://www.abcam.com/index.html?datasheet=17050).

    If you prefer two separate antibodies, I can recommend rabbit monoclonals over mouse monoclonals:

    http://www.epitomics.com/advantage/

    ab92331
    https://www.abcam.com/index.html?datasheet=92331 (or use the following: https://www.abcam.com/index.html?datasheet=92331).

    ab92507
    https://www.abcam.com/index.html?datasheet=92507 (or use the following: https://www.abcam.com/index.html?datasheet=92507).


    I hope this information is helpful. Should you have any further questions please do not hesitate to ask.

    Read More

    Answer

    Thank you for your inquiry.

    All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. These two products have been tested and are guaranteed to work in western blot on the species listed on the datasheet. The recommended concentrations in WB for EPR3554 and EPR3555 are 1:2000-1:10,000 and 1:1000-1:5000 respectively. However we recommend that users titer the optimal concentration for their experiments as these may lie outside of theis recommended range.

    The datsheets for these products may be found at the following locations:

    https://www.abcam.com/mrp8-antibody-epr3554-ab92331.html

    https://www.abcam.com/index.html?datasheet=92507

    Should you decide to go ahead and purchase this product, we encourage you to submit an Abreview of your results. We appreciate all customer feedback, whether positive or negative, and we make all product information available to researchers.

    To find out more about our Abreview system, please see the following webpage:

    https://www.abcam.com/abreviews

    Please do not hesitate to contact us again if you need further assistance.

    Read More

    Answer

    Thank you for your interest in our products. The net weight of the products is as follows:

    ab83534 Rabbit polyclonal to Psoriasin - 100 µg

    ab92331 Rabbit monoclonal to MRP8 - 100 µl

    ab92507 Rabbit monoclonal to S100A9 - 100 µl

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Answer

    Thank you very much for your consultation. It is rather difficult to compare the antibodies for effectiveness. Please note that the application notes on the datasheet are only an indication and can vary depending on the laboratory and the settings. The antibodies have in general not been tested in parallel. As you have already mentioned to the customer, we do however guarantee all our antibodies to work in the cited applications and species. Please find here also my comments for the antibodies the customer is looking for. 1.)  CDKN1C I can recommend the ab4058. Not only gives this antibody the most dilutions (if following the recommendations on the datasheet - which however are only an indication and can vary according to the different users), but it has also been tested in many different other applications which can be useful for the future for the customer. This is also a very popular antibody , been cited already in at least 7 publications.  (  Click here (or use the following: https://www.abcam.com/index.html?datasheet=4058). ab4058  500µl at 0.1mg/ml used at 1/100 in Abreview  - gives 500 / ab3223 500ul use at 1/100 to 1/50 gives 250 / ab119699 100ul used at 1/100 gives 100 / ab75974 rabMab 100ul  use at 1/100 to 1/250 - gives 250  / ab80703  250µl at 1mg/ml used at 1/50  - gives 125 /  ab99888 250 ul used at 1/100 gives 250) 2.) Rad17 We only have the ab63509 which is actually against total Rad17 and tested in IHC on paraffin sections on human tissue. I can recommend this antibody. Click here (or use the following: https://www.abcam.com/index.html?datasheet=63509). 3.) S100A9 I can recommend the ab92507 as it has been used diluted up to 1/500. Furthermore it has also been tested in WB and ICC. Click here (or use the following: https://www.abcam.com/index.html?datasheet=92507).  ( ab92507 100ul at 1/250 - 1/500 / ab62227 100ul use at 1/100 - 1/200 / ab92468 100ul at  1/100 to  1/250 / ab118441 100ul at 1/200 / ab59416 100ul at 1/50 ) I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.  

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    Question
    Answer

    Thank you for contacting us. The concentration of lot number GR49053 is 0.431 mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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