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A customer has the following query regarding these antibodies; I had checked the site and saw that there were a number of options for these three and hoped that you could advise as to the most effective for IHC on paraffin/formalin fixed. I usually would go with a rabbit monoclonal or rabbit polyclonal. Is it possible to ask Abcam? CDKN1C
Asked on Dec 08 2011
Thank you very much for your consultation. It is rather difficult to compare the antibodies for effectiveness. Please note that the application notes on the datasheet are only an indication and can vary depending on the laboratory and the settings. The antibodies have in general not been tested in parallel. As you have already mentioned to the customer, we do however guarantee all our antibodies to work in the cited applications and species. Please find here also my comments for the antibodies the customer is looking for. 1.) CDKN1C I can recommend the ab4058. Not only gives this antibody the most dilutions (if following the recommendations on the datasheet - which however are only an indication and can vary according to the different users), but it has also been tested in many different other applications which can be useful for the future for the customer. This is also a very popular antibody , been cited already in at least 7 publications. ( Click here (or use the following: https://www.abcam.com/index.html?datasheet=4058). ab4058 500µl at 0.1mg/ml used at 1/100 in Abreview - gives 500 / ab3223 500ul use at 1/100 to 1/50 gives 250 / ab119699 100ul used at 1/100 gives 100 / ab75974 rabMab 100ul use at 1/100 to 1/250 - gives 250 / ab80703 250µl at 1mg/ml used at 1/50 - gives 125 / ab99888 250 ul used at 1/100 gives 250) 2.) Rad17 We only have the ab63509 which is actually against total Rad17 and tested in IHC on paraffin sections on human tissue. I can recommend this antibody. Click here (or use the following: https://www.abcam.com/index.html?datasheet=63509). 3.) S100A9 I can recommend the ab92507 as it has been used diluted up to 1/500. Furthermore it has also been tested in WB and ICC. Click here (or use the following: https://www.abcam.com/index.html?datasheet=92507). ( ab92507 100ul at 1/250 - 1/500 / ab62227 100ul use at 1/100 - 1/200 / ab92468 100ul at 1/100 to 1/250 / ab118441 100ul at 1/200 / ab59416 100ul at 1/50 ) I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.
Answered on Dec 08 2011