Recombinant
RabMAb

Recombinant Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free (ab227570)

Overview

  • Product name

    Anti-S100A9 antibody [EPR3555] - Low endotoxin, Azide free
    See all S100A9 primary antibodies
  • Description

    Rabbit monoclonal [EPR3555] to S100A9 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human S100A9 aa 1-100.

  • Positive control

    • Breast cancer, Human tonsil, Human spleen and peripheral blood lymphocytes lysates, Human breast carcinoma tissue and Human spleen tissue. IF: DU145 cell line FC: Jurkat cells
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab227570 is a PBS only buffer version of ab92507, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab92507 for information on protocols, dilutions, and image data.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties

Applications

Our Abpromise guarantee covers the use of ab227570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22125622
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • Tissue specificity

      Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • Cellular localization

      Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • Database links

    • Alternative names

      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Images

    • Anti-S100A9 antibody [EPR3555] (ab92507)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling S100A9 with ab92507 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
      This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

    • This IHC data was generated using the same anti-S100A9 antibody clone, EPR3555, in a different buffer formulation (cat# ab92507).

      ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

    • Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling S100A9 (red) with purified ab92507 at a 1/200 dilution (10ug/mL). Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).

    • ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92507, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92507).

    • This IHC data was generated using the same anti-S100A9 antibody clone, EPR3555, in a different buffer formulation (cat# ab92507).

      ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Langley SR  et al. Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques. J Clin Invest 127:1546-1560 (2017). IHC-P ; Human . Read more (PubMed: 28319050) »
    • Saygideger-Kont Y  et al. Ezrin Enhances EGFR Signaling and Modulates Erlotinib Sensitivity in Non-Small Cell Lung Cancer Cells. Neoplasia 18:111-20 (2016). Read more (PubMed: 26936397) »
    See all 9 Publications for this product

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