Product nameAnti-S100A9 antibody [EPR3556] - BSA and Azide free
See all S100A9 primary antibodies
DescriptionRabbit monoclonal [EPR3556] to S100A9 - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues near the C terminus of Human S100A9 (UniProt ID: P06702).
- WB: Human tonsil lysate and peripheral blood leukocyte cell lysate IHC-P: Human spleen tissue
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Our Abpromise guarantee covers the use of ab227571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10,000. Predicted molecular weight: 13 kDa.
Is unsuitable for ICC/IF or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Tissue specificityExpressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains.
modificationsPhosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
- Information by UniProt
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
This product has been referenced in:
- Wei J et al. Perinatal exposure to bisphenol A exacerbates nonalcoholic steatohepatitis-like phenotype in male rat offspring fed on a high-fat diet. J Endocrinol 222:313-25 (2014). Read more (PubMed: 25112833) »
- Junker H et al. Stage-Related Alterations in Renal Cell Carcinoma - Comprehensive Quantitative Analysis by 2D-DIGE and Protein Network Analysis. PLoS One 6:e21867 (2011). WB, IHC-P ; Human . Read more (PubMed: 21760917) »