Recombinant
RabMAb

Recombinant Anti-S100A9 antibody [EPR3556] - BSA and Azide free (ab227571)

Overview

  • Product name

    Anti-S100A9 antibody [EPR3556] - BSA and Azide free
    See all S100A9 primary antibodies
  • Description

    Rabbit monoclonal [EPR3556] to S100A9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human S100A9 (UniProt ID: P06702).

  • Positive control

    • WB: Human tonsil lysate and peripheral blood leukocyte cell lysate IHC-P: Human spleen tissue
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab227571 is a PBS only buffer version of ab92468, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab92468 for information on protocols, dilutions, and image data.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties

Applications

Our Abpromise guarantee covers the use of ab227571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/1000 - 1/10,000. Predicted molecular weight: 13 kDa.

    Is unsuitable for ICC/IF or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • Tissue specificity

      Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses.
    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization.
    • Cellular localization

      Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Information by UniProt
    • Database links

    • Alternative names

      • Leukocyte L1 complex heavy chain antibody
      • 60B8AG antibody
      • CAGB antibody
      • Calgranulin B antibody
      • Calgranulin-B antibody
      • Calprotectin L1H subunit antibody
      • CFAG antibody
      • CGLB antibody
      • Cystic fibrosis antigen B antibody
      • L1AG antibody
      • Leukocyte L1 complex heavy chain antibody
      • LIAG antibody
      • MAC387 antibody
      • MIF antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor-related protein 14 antibody
      • MRP 14 antibody
      • MRP-14 antibody
      • MRP14 antibody
      • Myeloid-related protein 14 antibody
      • NIF antibody
      • OTTHUMP00000015331 antibody
      • p14 antibody
      • Protein S100-A9 antibody
      • S100 A9 antibody
      • S100 calcium binding protein A9 antibody
      • S100 calcium binding protein A9 calgranulin B antibody
      • S100 calcium-binding protein A9 antibody
      • S100A9 antibody
      • S10A9_HUMAN antibody
      see all

    Images

    • This IHC data was generated using the same anti-S100A9 antibody clone, EPR3556, in a different buffer formulation (cat# ab92468).

      ab92468, at a 1/100 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Wei J  et al. Perinatal exposure to bisphenol A exacerbates nonalcoholic steatohepatitis-like phenotype in male rat offspring fed on a high-fat diet. J Endocrinol 222:313-25 (2014). Read more (PubMed: 25112833) »
    • Junker H  et al. Stage-Related Alterations in Renal Cell Carcinoma - Comprehensive Quantitative Analysis by 2D-DIGE and Protein Network Analysis. PLoS One 6:e21867 (2011). WB, IHC-P ; Human . Read more (PubMed: 21760917) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab227571.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up