Overview

  • Product name

    Anti-S100P antibody [EPR6142]
    See all S100P primary antibodies
  • Description

    Rabbit monoclonal [EPR6142] to S100P
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human S100P aa 1-100. The exact sequence is proprietary.

  • Positive control

    • BxPC3 cell lysate; Human pancreatic adenocarcinoma, placenta and spleen tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P 1/150 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF 1/150.
Flow Cyt Use a concentration of 1 µg/ml.

Target

  • Tissue specificity

    Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization

    Nucleus. Cytoplasm. Colocalizes with S100PBP in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MIG9 antibody
    • Migration inducing gene 9 antibody
    • Protein S100-E antibody
    • Protein S100-P antibody
    • Protein S100P antibody
    • S100 calcium binding protein P antibody
    • S100 calcium-binding protein P antibody
    • S100 P antibody
    • S100E antibody
    • S100P antibody
    • S100P_HUMAN antibody
    see all

Images

  • Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (purified) + BxPC-3 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry analysis of BxPC-3 (human Pancreas adenocarcinoma) cells labeling S100P with purified ab124743 at 1/1700 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemical staining of paraffin embedded human pancreatic adenocarcinoma with purified ab124743 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunofluorescence staining of BxPC-3 cells with purified ab124743 at a working dilution of 1 in 150, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti-rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab124743 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500.

  • Anti-S100P antibody [EPR6142] (ab124743) at 1/1000 dilution (unpurified) + BxPC3 cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

  • Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human pancreatic adenocarcinoma tissue by immunohistochemistry.

  • Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human placenta tissue by immunohistochemistry.

  • Unpurified ab124743, at 1/500 dilution, staining S100P in paraffin-embedded human spleen tissue by immunohistochemistry.

  • Unpurified ab124743 showing negative staining in normal liver tissue.

  • Unpurified ab124743 showing positive staining in colonic adenocarcinoma tissue.

  • Unpurified ab124743 showing negative staining in normal pancreas tissue.

References

This product has been referenced in:

  • Zhang X & Zhuang R Dione-thiophene conjugate inhibits proliferation and metastasis of nasopharyngeal carcinoma cells through calcium binding protein-P down-regulation. Eur J Med Chem 168:199-206 (2019). Read more (PubMed: 30822709) »
  • Liu Y  et al. S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett 14:525-532 (2017). Read more (PubMed: 28693201) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thanks for contacting us. The immunogen for ab124743 Anti-S100P, has 56% sequence similarity with S100A1, and 33% with S100B. Therefore, it is unlikely that the antibody will cross react with either of these proteins.

I hope this information is helpful. If you have any other questions please let me know. Have a good day.

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