Overview

  • Product name

  • Description

    Rabbit polyclonal to S1P
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Horse, Chicken, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human S1P aa 200-300 conjugated to keyhole limpet haemocyanin.
    Database link: Q14703
    (Peptide available as ab223083)

  • Positive control

    • WB: Mouse and rat liver tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab140592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human S1P peptide (ab223083).

Target

  • Relevance

    S1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain.
  • Cellular localization

    Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein.
  • Database links

  • Alternative names

    • kexin isozyme 1 antibody
    • KIAA0091 antibody
    • MBTPS1 antibody
    • membrane bound transcription factor peptidase, site 1 antibody
    • MGC138711 antibody
    • MGC138712 antibody
    • PCSK8 antibody
    • S1P antibody
    • S1P endopeptidase antibody
    • Site 1 protease antibody
    • SKI 1 antibody
    • SKI1 antibody
    • Subtilisin isozyme 1 antibody
    • Subtilisin/kexin isozyme 1 antibody
    see all

Images

  • All lanes : Anti-S1P antibody (ab140592) at 1 µg/ml

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Rat liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 118 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 62 kDa (possible non-specific binding)


    Exposure time: 90 seconds


    The band observed at 100 kDa could potentially be a cleaved form of S1P due to the presence of a 17 amino acid signal peptide and a 169 amino acid pro-peptide.

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140592 overnight at 4°C.

References

ab140592 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 14 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Fatty liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Fatty liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 25 2016

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