Product nameAnti-S1P antibody
See all S1P primary antibodies
DescriptionRabbit polyclonal to S1P
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse, Chicken, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
- WB: Mouse and rat liver tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab140592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human S1P peptide (ab223083).|
RelevanceS1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain.
Cellular localizationEndoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein.
- kexin isozyme 1 antibody
- KIAA0091 antibody
- MBTPS1 antibody
All lanes : Anti-S1P antibody (ab140592) at 1 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa (possible non-specific binding)
Exposure time: 90 seconds
The band observed at 100 kDa could potentially be a cleaved form of S1P due to the presence of a 17 amino acid signal peptide and a 169 amino acid pro-peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140592 overnight at 4°C.
ab140592 has not yet been referenced specifically in any publications.