Immunocytochemistry/ Immunofluorescence abreview for Anti-S1P antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in 0.1% PBST
Dilution
1/50

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-Rb
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

Additional data

Abcam response
Thank you for submitting this AbReview. This antibody has so far not been tested with rat samples or in Immunocytochemistry yet. We would like to encourage other researchers to submit an Abreview with their rat samples when this antibody had been used.

Dr. Armen Petrosyan

Verified customer

Submitted Dec 21 2016

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