- Datasheet
- Specific References (8)
- Protocols
Overview
-
Product nameAnti-S1P1/EDG1 antibody
See all S1P1/EDG1 primary antibodies -
DescriptionRabbit polyclonal to S1P1/EDG1
-
Host speciesRabbit
-
Specificityab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
-
Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Cow, Cat, Monkey
-
Immunogen
Synthetic peptide corresponding to Mouse S1P1/EDG1 aa 359-372.
Sequence:SHPQKDDGDNPETI
(Peptide available asab39763) -
Positive control
- Transfected HEK293 cells.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab11424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa.Can be blocked with Human S1P1/EDG1 peptide (ab39763). Band represents recombinant S1P1 from transfected HEK293 cells. | |
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 20733200 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | Use at an assay dependent concentration. |
Target
-
FunctionReceptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
-
Tissue specificityEndothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
-
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
-
Post-translational
modificationsS1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
Cellular localizationCell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 281135 Cow
- Entrez Gene: 100050049 Horse
- Entrez Gene: 1901 Human
- Entrez Gene: 13609 Mouse
- Entrez Gene: 29733 Rat
- Omim: 601974 Human
- SwissProt: Q5E9P3 Cow
- SwissProt: P21453 Human
see all -
Alternative names
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
Images
-
Western blot - Anti-S1P1/EDG1 antibody (ab11424)Lane 1 : Anti-S1P1/EDG1 antibody (ab11424) at 2 µg/ml
Lane 2 : Anti-S1P1/EDG1 antibody (ab11424) at 2 mg/ml
Lane 1 : Rat brain tissue extract
Lane 2 : Neuro-2a Cells
Secondary
All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml
Developed using the ECL technique. -
Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.
Green staining - ab11424
Blue staining - DAPI for nuclear localization
Red staining - F-actin
The right-most image is a control (no primary antibody staining).
-
Flow Cytometry - Anti-S1P1/EDG1 antibody (ab11424)Flow cytometry analysis of SH-SY5Y cells, labeling S1P1/EDG1 with ab11424 (3-5µg/million cells in 2.5% BSA). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.
Red histogram - labeling with anti-S1P1/EDG1 antibody
Purple histogram - Unstained
Green histogram - No primary antibody control
Pink histogram - Rabbit Isotype control
-
Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.
Protocols
References
This product has been referenced in:
- Hamidi Shishavan M et al. Differential Effects of Long Term FTY720 Treatment on Endothelial versus Smooth Muscle Cell Signaling to S1P in Rat Mesenteric Arteries. PLoS One 11:e0162029 (2016). Read more (PubMed: 27583547) »
- Alfonso J et al. Downregulation of Sphingosine 1-Phosphate Receptor 1 Promotes the Switch from Tangential to Radial Migration in the OB. J Neurosci 35:13659-72 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 26446219) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"