Overview

  • Product name
  • Description
    Rabbit polyclonal to S1P1/EDG1
  • Host species
    Rabbit
  • Specificity
    ab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Cat, Monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse S1P1/EDG1 aa 359-372.
    Sequence:

    SHPQKDDGDNPETI


    (Peptide available as ab39763)

  • Positive control
    • Transfected HEK293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab11424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa.Can be blocked with Human S1P1/EDG1 peptide (ab39763). Band represents recombinant S1P1 from transfected HEK293 cells.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20733200

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
  • Tissue specificity
    Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD363 antibody
    • CHEDG 1 antibody
    • CHEDG1 antibody
    • D1S3362 antibody
    • ECGF 1 antibody
    • ECGF1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • endothelial differentiation G protein coupled receptor 1 antibody
    • Endothelial differentiation G-protein coupled receptor 1 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 1 antibody
    • FLJ58121 antibody
    • G protein coupled sphingolipid receptor antibody
    • g protein-coupled receptor edg-1 antibody
    • S1P receptor 1 antibody
    • S1P receptor Edg 1 antibody
    • S1P receptor Edg-1 antibody
    • S1P receptor Edg1 antibody
    • S1P(1) receptor antibody
    • S1P1 antibody
    • s1pr1 antibody
    • S1PR1_HUMAN antibody
    • sphingolipid g-protein-coupled receptor 1 antibody
    • Sphingosine 1 phosphate receptor Edg 1 antibody
    • Sphingosine 1 phosphate receptor EDG1 antibody
    • sphingosine 1- phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor Edg-1 antibody
    • Sphingosine 1phosphate receptor type 1 S1P1 antibody
    see all

Images

  • Lane 1 : Anti-S1P1/EDG1 antibody (ab11424) at 2 µg/ml
    Lane 2 : Anti-S1P1/EDG1 antibody (ab11424) at 2 mg/ml

    Lane 1 : Rat brain tissue extract
    Lane 2 : Neuro-2a Cells

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml

    Developed using the ECL technique.
  • Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.

    Green staining - ab11424

    Blue staining - DAPI for nuclear localization 

    Red staining - F-actin

    The right-most image is a control (no primary antibody staining).

  • Flow cytometry analysis of SH-SY5Y cells, labeling S1P1/EDG1 with ab11424 (3-5µg/million cells in 2.5% BSA). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.

    Red histogram - labeling with anti-S1P1/EDG1 antibody

    Purple histogram - Unstained

    Green histogram - No primary antibody control

    Pink histogram - Rabbit Isotype control

  • ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.

References

This product has been referenced in:
  • Wang M  et al. Apolipoprotein M induces inhibition of inflammatory responses via the S1PR1 and DHCR24 pathways. Mol Med Rep 19:1272-1283 (2019). Read more (PubMed: 30569161) »
  • Liu S  et al. S1PR1 regulates the switch of two angiogenic modes by VE-cadherin phosphorylation in breast cancer. Cell Death Dis 10:200 (2019). Read more (PubMed: 30814488) »
See all 13 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Western blot
Sample
Sheep Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (Bolt 4-12% Bis-Tris)
Loading amount
10 µg
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Amanda Rice

Verified customer

Submitted May 09 2018

Question
Answer



I am very pleased to hear you would like to accept our offer and test ab11424 in rat. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for contacting us and I am sorry to hear that ab11424 is not providing satisfactory results. I am going to refund this purchase for you. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

Read More

Answer

Thank you for making us aware that you experienced a problem with our EDG1 antibody ab11424. As my colleague mentioned, this product is guaranteed to work with mouse samples in WB and can be replaced or refunded per our Abpromise.  For our records, I would like to gather some additional information about the protocol you tested and your samples.  Can you please provide a protocol summary and an image of your results?  We will use this information to investigate this problem further. Should we determine that the antibody is not working as stated on the datasheet, I would be happy to offer a replacement or credit.  Please let me know which you would prefer. I look forward to your reply so that I may assist you further.  Please do not hesitate to contact us if you have any additonal questions.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal Cord)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
80 µg
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 21 2011

Question
Answer

Thank you for your inquiry. I heard back from the testing laboratory regarding the fixation/permeabilization protocol for ab11424. Aspirate culture medium and add warmed Fixation Solution (4% Formaldehyde, 37C) and incubate 15 minutes at room temperature. Aspirate Fixation Solution and wash with Dulbecco’s PBS. Aspirate solution. Add Permeabilization Solution (Dulbecco’s PBS with 0.1% Trition X100) and incubate 15 minutes at room temperature. Aspirate solution and proceed to blocking/antibody steps. I hope this information helps. I hope to have the ICC/IF image on the datasheet shortly. Please contact us with any other questions.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Dr. Francesca Cencetti

Verified customer

Submitted Oct 06 2010

Answer

Thank you for your email. When ab11424 was tested in IF, transfected CCL39 cells were used. So to our knowledge it has not yet been tested in IHC. Ab13126 has only been tested for cross-reactivity with human and we don't have any information about how it will work with other species. Neither antibody has been tested yet for application in Flow Cytometry. If you have any more questions, please contact us again.

Read More

Answer

Thank you very much for your patience, I have just heard back from the originator of ab11424. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. The figure on the website shows a Western blot of S1P1 in transfected HEK293 cell lysate using ab11424; the band is at approximately 44 kDa. It is recommended to use transfected HEK293 cells as a positive control. If you need further assistance with this antibody, please do let me know.

Read More

Answer

HEK293 cells were transiently transfected with the myc-tagged receptor. After 48 hrs, cells were solubilized (in buffer containing: 1% triton X-100, 0.5% deoxycholate, 0.1% SDS.) and protein-equalized. Samples were fractionated by SDS-PAGE (12% gel). After transfer to nitrocellulose, membrane was incubated overnight at 4 deg. C with ab11424 followed by HRP-conjugated anti-rabbit IgG.

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1-10 of 11 Abreviews or Q&A

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