For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    s1p1edg1-antibody-ab11424.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Receptors Associated Proteins
Share by email

Anti-S1P1/EDG1 antibody (ab11424)

  • Datasheet
  • SDS
Reviews (3)Q&A (8)References (25)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-S1P1/EDG1 antibody (ab11424)
  • Immunocytochemistry - Anti-S1P1/EDG1 antibody (ab11424)
  • Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)

Key features and details

  • Rabbit polyclonal to S1P1/EDG1
  • Suitable for: IP, ICC/IF, IHC-P, WB, Flow Cyt, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-S1P1/EDG1 antibody [EPR21202] (ab233386)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-S1P1/EDG1 antibody
    See all S1P1/EDG1 primary antibodies
  • Description

    Rabbit polyclonal to S1P1/EDG1
  • Host species

    Rabbit
  • Specificity

    ab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WB, Flow Cyt, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Cat, Monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse S1P1/EDG1 aa 359-372.
    Sequence:

    SHPQKDDGDNPETI


    (Peptide available as ab39763)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Rat brain tissue lysate; Neuro-2a cell lysate. ICC: CCL39 and Neuro-2a cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human S1P1/EDG1 protein (ab132072)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa. Band represents recombinant S1P1 from transfected HEK293 cells.
Flow Cyt
Use at an assay dependent concentration. PubMed: 20733200

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC
Use a concentration of 2 µg/ml.
Notes
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa. Band represents recombinant S1P1 from transfected HEK293 cells.
Flow Cyt
Use at an assay dependent concentration. PubMed: 20733200

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC
Use a concentration of 2 µg/ml.

Target

  • Function

    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
  • Tissue specificity

    Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P21453 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281135 Cow
    • Entrez Gene: 100050049 Horse
    • Entrez Gene: 1901 Human
    • Entrez Gene: 13609 Mouse
    • Entrez Gene: 29733 Rat
    • Omim: 601974 Human
    • SwissProt: Q5E9P3 Cow
    • SwissProt: P21453 Human
    • SwissProt: O08530 Mouse
    • SwissProt: P48303 Rat
    • Unigene: 154210 Human
    • Unigene: 982 Mouse
    • Unigene: 109455 Rat
    see all
  • Alternative names

    • CD363 antibody
    • CHEDG 1 antibody
    • CHEDG1 antibody
    • D1S3362 antibody
    • ECGF 1 antibody
    • ECGF1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • endothelial differentiation G protein coupled receptor 1 antibody
    • Endothelial differentiation G-protein coupled receptor 1 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 1 antibody
    • FLJ58121 antibody
    • G protein coupled sphingolipid receptor antibody
    • g protein-coupled receptor edg-1 antibody
    • S1P receptor 1 antibody
    • S1P receptor Edg 1 antibody
    • S1P receptor Edg-1 antibody
    • S1P receptor Edg1 antibody
    • S1P(1) receptor antibody
    • S1P1 antibody
    • s1pr1 antibody
    • S1PR1_HUMAN antibody
    • sphingolipid g-protein-coupled receptor 1 antibody
    • Sphingosine 1 phosphate receptor Edg 1 antibody
    • Sphingosine 1 phosphate receptor EDG1 antibody
    • sphingosine 1- phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor Edg-1 antibody
    • Sphingosine 1phosphate receptor type 1 S1P1 antibody
    see all

Images

  • Western blot - Anti-S1P1/EDG1 antibody (ab11424)
    Western blot - Anti-S1P1/EDG1 antibody (ab11424)
    Lane 1 : Anti-S1P1/EDG1 antibody (ab11424) at 2 µg/ml
    Lane 2 : Anti-S1P1/EDG1 antibody (ab11424) at 2 mg/ml

    Lane 1 : Rat brain tissue extract
    Lane 2 : Neuro-2a Cells

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml

    Developed using the ECL technique.
  • Immunocytochemistry - Anti-S1P1/EDG1 antibody (ab11424)
    Immunocytochemistry - Anti-S1P1/EDG1 antibody (ab11424)

    Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.

    Green staining - ab11424

    Blue staining - DAPI for nuclear localization 

    Red staining - F-actin

    The right-most image is a control (no primary antibody staining).

  • Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)
    Immunocytochemistry/ Immunofluorescence - Anti-S1P1/EDG1 antibody (ab11424)

    ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (25)

Publishing research using ab11424? Please let us know so that we can cite the reference in this datasheet.

ab11424 has been referenced in 25 publications.

  • Wang Z  et al. Mfsd2a and Spns2 are essential for sphingosine-1-phosphate transport in the formation and maintenance of the blood-brain barrier. Sci Adv 6:eaay8627 (2020). PubMed: 32523984
  • Xie Y  et al. miR-363 suppresses the proliferation, migration and invasion of clear cell renal cell carcinoma by downregulating S1PR1. Cancer Cell Int 20:227 (2020). PubMed: 32536815
  • Chen SL  et al. JAK/STAT signaling pathway-mediated microRNA-181b promoted blood-brain barrier impairment by targeting sphingosine-1-phosphate receptor 1 in septic rats. Ann Transl Med 8:1458 (2020). PubMed: 33313203
  • Matsumoto N  et al. Up-regulation of sphingosine-1-phosphate receptors and sphingosine kinase 1 in the peri-ischemic area after transient middle cerebral artery occlusion in mice. Brain Res 1739:146831 (2020). PubMed: 32278723
  • Zou Y  et al. Non-Mitogenic Fibroblast Growth Factor 1 Enhanced Angiogenesis Following Ischemic Stroke by Regulating the Sphingosine-1-Phosphate 1 Pathway. Front Pharmacol 11:59 (2020). PubMed: 32194396
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-S1P1/EDG1 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Sheep Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (Bolt 4-12% Bis-Tris)
Loading amount
10 µg
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Amanda Rice

Verified customer

Submitted May 09 2018

Western blot abreview for Anti-S1P1/EDG1 antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal Cord)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
80 µg
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 21 2011

Western blot abreview for Anti-S1P1/EDG1 antibody

Average
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Francesca Cencetti

Verified customer

Submitted Oct 06 2010

Question

I would like to test ab11424 with rat samples in western blotting.

Read More

Abcam community

Verified customer

Asked on Feb 22 2012

Answer



I am very pleased to hear you would like to accept our offer and test ab11424 in rat. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Feb 22 2012

Question

This antibody is showing bands in both my WT and KO mouse model.

Read More

Abcam community

Verified customer

Asked on Oct 27 2011

Answer

Thank you for contacting us and I am sorry to hear that ab11424 is not providing satisfactory results. I am going to refund this purchase for you. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

Read More

Abcam Scientific Support

Answered on Oct 27 2011

Question

I purchased ab11424 a Rabbit Polyclonal to EDG1, and used it in WB and mouse which seems to be guaranteed on the datasheet. I had very weak bands and had better results with a similar ab from another company.

Read More

Abcam community

Verified customer

Asked on Oct 27 2011

Answer

Thank you for making us aware that you experienced a problem with our EDG1 antibody ab11424. As my colleague mentioned, this product is guaranteed to work with mouse samples in WB and can be replaced or refunded per our Abpromise.  For our records, I would like to gather some additional information about the protocol you tested and your samples.  Can you please provide a protocol summary and an image of your results?  We will use this information to investigate this problem further. Should we determine that the antibody is not working as stated on the datasheet, I would be happy to offer a replacement or credit.  Please let me know which you would prefer. I look forward to your reply so that I may assist you further.  Please do not hesitate to contact us if you have any additonal questions.

Read More

Abcam Scientific Support

Answered on Oct 27 2011

Question

How were the cells fixed for ICC/IF using this antibody? I would also like to see an image.

Read More

Abcam community

Verified customer

Asked on May 16 2011

Answer

Thank you for your inquiry. I heard back from the testing laboratory regarding the fixation/permeabilization protocol for ab11424. Aspirate culture medium and add warmed Fixation Solution (4% Formaldehyde, 37C) and incubate 15 minutes at room temperature. Aspirate Fixation Solution and wash with Dulbecco’s PBS. Aspirate solution. Add Permeabilization Solution (Dulbecco’s PBS with 0.1% Trition X100) and incubate 15 minutes at room temperature. Aspirate solution and proceed to blocking/antibody steps. I hope this information helps. I hope to have the ICC/IF image on the datasheet shortly. Please contact us with any other questions.

Read More

Abcam Scientific Support

Answered on May 16 2011

Question

This enquiry is in regard to EDG1 antibodies (both ab11424 and ab13126). Ultimately we would like to use an EDG1 antibody to stain formalin-fixed paraffin embedded tissue sections of rhesus macaques (monkeys). The product sheet for ab11424 states that the antibody can be used for immunofluorescence, but does this include immunofluorescent detection on tissue sections (fixed, frozen or paraffin embedded)? Ab13126 appears to work in formalin-fixed paraffin-embedded sections, but has not been tested in any other species except humans, is this antibody expected to cross-react with a broad range of species like the ab11424 antibody? Also, could either of these antibodies be used to stain unfixed cells for flow cytometric analysis?

Read More

Abcam community

Verified customer

Asked on Oct 21 2004

Answer

Thank you for your email. When ab11424 was tested in IF, transfected CCL39 cells were used. So to our knowledge it has not yet been tested in IHC. Ab13126 has only been tested for cross-reactivity with human and we don't have any information about how it will work with other species. Neither antibody has been tested yet for application in Flow Cytometry. If you have any more questions, please contact us again.

Read More

Abcam Scientific Support

Answered on Oct 27 2004

Question

All I want to know is: I've used the anti-Edg-1 mAb ab11424 on mouse liver lysate in a standard western. I got a nice clear and specific band, but it's not at 44kDa like your data sheet suggests. The picture on your website shows a western blot, but does not say where the band is on the gel i.e. it's size.

Read More

Abcam community

Verified customer

Asked on Jun 30 2004

Answer

Thank you very much for your patience, I have just heard back from the originator of ab11424. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. The figure on the website shows a Western blot of S1P1 in transfected HEK293 cell lysate using ab11424; the band is at approximately 44 kDa. It is recommended to use transfected HEK293 cells as a positive control. If you need further assistance with this antibody, please do let me know.

Read More

Abcam Scientific Support

Answered on Jul 12 2004

Question

Could I please have some information on how the protein lysates were prepared to test the anti-Edg1 antibody. Were the samples tested on NP-40 lysates, cell pallets, membrane preps ...? What detailed procedure was used.

Read More

Abcam community

Verified customer

Asked on Jun 18 2004

Answer

HEK293 cells were transiently transfected with the myc-tagged receptor. After 48 hrs, cells were solubilized (in buffer containing: 1% triton X-100, 0.5% deoxycholate, 0.1% SDS.) and protein-equalized. Samples were fractionated by SDS-PAGE (12% gel). After transfer to nitrocellulose, membrane was incubated overnight at 4 deg. C with ab11424 followed by HRP-conjugated anti-rabbit IgG.

Read More

Abcam Scientific Support

Answered on Jun 21 2004

1-10 of 11 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.