Overview

  • Product name
  • Description
    Rabbit polyclonal to S1P1/EDG1
  • Host species
    Rabbit
  • Specificity
    ab11424 detects S1P1/EDG1 protein in transfected human cell samples. This antibody shows no cross-reactivity to S1P3.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Cat, Monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse S1P1/EDG1 aa 359-372.
    Sequence:

    SHPQKDDGDNPETI


    (Peptide available as ab39763)

  • Positive control
    • Transfected HEK293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab11424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa.Can be blocked with Human S1P1/EDG1 peptide (ab39763). Band represents recombinant S1P1 from transfected HEK293 cells.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20733200

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
  • Tissue specificity
    Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD363 antibody
    • CHEDG 1 antibody
    • CHEDG1 antibody
    • D1S3362 antibody
    • ECGF 1 antibody
    • ECGF1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • endothelial differentiation G protein coupled receptor 1 antibody
    • Endothelial differentiation G-protein coupled receptor 1 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 1 antibody
    • FLJ58121 antibody
    • G protein coupled sphingolipid receptor antibody
    • g protein-coupled receptor edg-1 antibody
    • S1P receptor 1 antibody
    • S1P receptor Edg 1 antibody
    • S1P receptor Edg-1 antibody
    • S1P receptor Edg1 antibody
    • S1P(1) receptor antibody
    • S1P1 antibody
    • s1pr1 antibody
    • S1PR1_HUMAN antibody
    • sphingolipid g-protein-coupled receptor 1 antibody
    • Sphingosine 1 phosphate receptor Edg 1 antibody
    • Sphingosine 1 phosphate receptor EDG1 antibody
    • sphingosine 1- phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor Edg-1 antibody
    • Sphingosine 1phosphate receptor type 1 S1P1 antibody
    see all

Images

  • Lane 1 : Anti-S1P1/EDG1 antibody (ab11424) at 2 µg/ml
    Lane 2 : Anti-S1P1/EDG1 antibody (ab11424) at 2 mg/ml

    Lane 1 : Rat brain tissue extract
    Lane 2 : Neuro-2a Cells

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 0.4 µg/ml

    Developed using the ECL technique.
  • Immunocytochemical analysis of Neuro-2a cells, labeling S1P1/EDG1 with ab11424. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Immunostaining with anti-EDG1 diluted 2µg/ml in 0.1% BSA for 3 hours at room temperature.

    Green staining - ab11424

    Blue staining - DAPI for nuclear localization 

    Red staining - F-actin

    The right-most image is a control (no primary antibody staining).

  • Flow cytometry analysis of SH-SY5Y cells, labeling S1P1/EDG1 with ab11424 (3-5µg/million cells in 2.5% BSA). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes and blocked with 5% BSA for 30 minutes at room temperature.

    Red histogram - labeling with anti-S1P1/EDG1 antibody

    Purple histogram - Unstained

    Green histogram - No primary antibody control

    Pink histogram - Rabbit Isotype control

  • ab11424 staining S1P1/EDG1 in transfected CCL39 cells by Immunocytochemistry/ Immunofluorescence.

References

This product has been referenced in:
  • Wang M  et al. Apolipoprotein M induces inhibition of inflammatory responses via the S1PR1 and DHCR24 pathways. Mol Med Rep 19:1272-1283 (2019). Read more (PubMed: 30569161) »
  • Liu S  et al. S1PR1 regulates the switch of two angiogenic modes by VE-cadherin phosphorylation in breast cancer. Cell Death Dis 10:200 (2019). Read more (PubMed: 30814488) »
See all 13 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Sheep Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (Bolt 4-12% Bis-Tris)
Loading amount
10 µg
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Amanda Rice

Verified customer

Submitted May 09 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal Cord)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
80 µg
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 21 2011

Application
Western blot
Sample
Mouse Cell lysate - whole cell (skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Dr. Francesca Cencetti

Verified customer

Submitted Oct 06 2010

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