Anti-S1P1/EDG1 antibody (ab140952)
Key features and details
- Rabbit polyclonal to S1P1/EDG1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-S1P1/EDG1 antibody
See all S1P1/EDG1 primary antibodies -
Description
Rabbit polyclonal to S1P1/EDG1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Hamster, Cow, Dog, Turkey, Pig, Xenopus laevis, Non human primates, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human S1P1/EDG1. Corresponds to a 16 residue sequence within the first cytoplasmic domain.
Database link: NP_001391.2 -
Positive control
- Human heart (vessel) tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. -
Tissue specificity
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsS1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 281135 Cow
- Entrez Gene: 100050049 Horse
- Entrez Gene: 1901 Human
- Entrez Gene: 13609 Mouse
- Entrez Gene: 29733 Rat
- Entrez Gene: 444659 Xenopus laevis
- Entrez Gene: 780355 Xenopus tropicalis
- Omim: 601974 Human
see all -
Alternative names
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab140952 has not yet been referenced specifically in any publications.