Overview

  • Product name

  • Description

    Rabbit polyclonal to S1P1/EDG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Hamster, Cow, Dog, Turkey, Pig, Xenopus laevis, Non human primates, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human S1P1/EDG1. Corresponds to a 16 residue sequence within the first cytoplasmic domain.
    Database link: NP_001391.2

  • Positive control

    • Human heart (vessel) tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab140952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
  • Tissue specificity

    Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD363 antibody
    • CHEDG 1 antibody
    • CHEDG1 antibody
    • D1S3362 antibody
    • ECGF 1 antibody
    • ECGF1 antibody
    • EDG 1 antibody
    • EDG1 antibody
    • endothelial differentiation G protein coupled receptor 1 antibody
    • Endothelial differentiation G-protein coupled receptor 1 antibody
    • Endothelial differentiation sphingolipid G protein coupled receptor 1 antibody
    • FLJ58121 antibody
    • G protein coupled sphingolipid receptor antibody
    • g protein-coupled receptor edg-1 antibody
    • S1P receptor 1 antibody
    • S1P receptor Edg 1 antibody
    • S1P receptor Edg-1 antibody
    • S1P receptor Edg1 antibody
    • S1P(1) receptor antibody
    • S1P1 antibody
    • s1pr1 antibody
    • S1PR1_HUMAN antibody
    • sphingolipid g-protein-coupled receptor 1 antibody
    • Sphingosine 1 phosphate receptor Edg 1 antibody
    • Sphingosine 1 phosphate receptor EDG1 antibody
    • sphingosine 1- phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor 1 antibody
    • Sphingosine 1-phosphate receptor Edg-1 antibody
    • Sphingosine 1phosphate receptor type 1 S1P1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart (vessel) tissue labeling S1P1/EDG1 with ab140952 at 6 μg/ml.

References

ab140952 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Sheep Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (Bolt 4-12% Bis-Tris Plus gel 15-well)
Loading amount
10 µg
Specification
lung
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Amanda Rice

Verified customer

Submitted May 09 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (Bolt 4-12% Bis-Tris Plus gel 15-well)
Loading amount
10 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Amanda Rice

Verified customer

Submitted May 09 2018

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