Recombinant Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)
![Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)](https://www.abcam.com/ps/products/259/ab259902/Images/ab259902-467219-anti-s1p1edg1-antibody-epr23535146-western-blot-lysate.jpg "Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23535-146] to S1P1/EDG1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-S1P1/EDG1 antibody [EPR23535-146]
See all S1P1/EDG1 primary antibodies -
Description
Rabbit monoclonal [EPR23535-146] to S1P1/EDG1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse brain, Mouse lung, bEnd.3 and Rat brain lysates. IHC-P: Mouse cerebrum, Rat cerebrum tissues. IP: Mouse brain tissue lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23535-146 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab259902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
|
| IHC-P |
1/100.
|
|
| WB |
1/1000. Predicted molecular weight: 43 kDa.
|
| Notes |
|---|
|
IP
1/30. |
|
IHC-P
1/100. |
|
WB
1/1000. Predicted molecular weight: 43 kDa. |
Target
-
Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins. -
Tissue specificity
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsS1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 13609 Mouse
- Entrez Gene: 29733 Rat
- SwissProt: O08530 Mouse
- SwissProt: P48303 Rat
- Unigene: 982 Mouse
- Unigene: 109455 Rat
-
Alternative names
- CD363 antibody
- CHEDG 1 antibody
- CHEDG1 antibody
see all
Images
-
Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)All lanes : Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse lung tissue lysate 20
Lane 3 : Mouse testis tissue lysate
Lane 4 : bEnd.3 (mouse brain endothelioma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 43 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The molecular weight observed is consistent with what has been described in the literature (PMID:19810093, 14688232).
Low expression: testis (PMID:9931453).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 48 seconds
-
![Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)](https://www.abcam.com/ps/products/259/ab259902/Images/ab259902-467220-anti-s1p1edg1-antibody-epr23535146-western-blot-lysate1.jpg)
Western blot - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)All lanes : Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 43 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: testis (PMID:9931453).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 48 seconds
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)](https://www.abcam.com/ps/products/259/ab259902/Images/ab259902-1-anti-s1p1edg1-antibody-epr23535146-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling S1P1/EDG1 with ab259902 at 1/100 (5.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in mouse cerebrum. The section was incubated with ab254360 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)](https://www.abcam.com/ps/products/259/ab259902/Images/ab259902-2-anti-s1p1edg1-antibody-epr23535146-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling S1P1/EDG1 with ab259902 at 1/100 (5.08 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in rat cerebrum. The section was incubated with ab254360 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
-
![Immunoprecipitation - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)](https://www.abcam.com/ps/products/259/ab259902/Images/ab259902-3-anti-s1p1edg1-antibody-epr23535146-immunoprecipitation-mouse-brain-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)S1P1/EDG1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab259902 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259902 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab259902 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259902 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5.5 seconds
Sample loaded onto lane 1 is non-boiled as boiling may cause protein aggregates.
-
![Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)](https://www.abcam.com/ps/products/259/ab259902/Images/ab259902-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-S1P1/EDG1 antibody [EPR23535-146] (ab259902)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab259902 has not yet been referenced specifically in any publications.