Anti-S1PR2 antibody (ab235919)
Key features and details
- Rabbit polyclonal to S1PR2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S1PR2 antibody
See all S1PR2 primary antibodies -
Description
Rabbit polyclonal to S1PR2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human S1PR2 aa 250 to the C-terminus.
Database link: O95136 -
Positive control
- WB: MCF-7, 293, and Ntera-2 whole cell lysates. ICC/IF: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/5000. Predicted molecular weight: 39 kDa.
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ICC/IF |
1/200 - 1/500.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 39 kDa. |
ICC/IF
1/200 - 1/500. |
Target
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Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9294 Human
- Entrez Gene: 29415 Rat
- Omim: 605111 Human
- SwissProt: O95136 Human
- SwissProt: P47752 Rat
- Unigene: 655405 Human
- Unigene: 2491 Rat
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Alternative names
- AGR16 antibody
- EDG 5 antibody
- EDG5 antibody
see all
Images
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All lanes : Anti-S1PR2 antibody (ab235919) at 1/500 dilution
Lane 1 : MCF-7 whole cell lysate
Lane 2 : 293 whole cell lysate
Lane 3 : Ntera-2 whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for S1PR2 (green) using ab235919 at 1/200 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Counter stained with DAPI.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab235919 has been referenced in 3 publications.
- Lin Q et al. Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Exp Ther Med 23:3 (2022). PubMed: 34815755
- Liu JX et al. Clonorchis sinensis infection induces hepatobiliary injury via disturbing sphingolipid metabolism and activating sphingosine 1-phosphate receptor 2. Front Cell Infect Microbiol 12:1011378 (2022). PubMed: 36339341
- Li Y et al. Sphingosine-1-phosphate receptor 2 modulates pain sensitivity by suppressing the ROS-RUNX3 pathway in a rat model of neuropathy. J Cell Physiol 235:3864-3873 (2020). PubMed: 31603252