Key features and details
- Rabbit polyclonal to S1PR2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-S1PR2 antibody
DescriptionRabbit polyclonal to S1PR2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- WB: Mouse brain lysate. IHC-P: Human small intestine tissue. ICC/IF: SH-SY5Y cells.
This product was previously labelled as EDG5
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/200 - 1/500.|
FunctionReceptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- AGR16 antibody
- EDG 5 antibody
- EDG5 antibody
Paraffin-embedded human small intestine tissue stained for S1PR2 using ab235919 at 1/100 dilution in immunohistochemical analysis.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for S1PR2 (green) using ab235919 at 1/200 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). Counter stained with DAPI.
Anti-S1PR2 antibody (ab235919) at 1/500 dilution + Mouse brain lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235919 has not yet been referenced specifically in any publications.