• Product name

  • Description

    Rabbit polyclonal to S2P
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human S2P aa 300-400 conjugated to keyhole limpet haemocyanin.
    Database link: O43462

  • Positive control

    • This antibody gave a positive signal in Human Heart and Liver tissue lysates.



Our Abpromise guarantee covers the use of ab140594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).


  • Function

    Intramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
  • Involvement in disease

    Defects in MBTPS2 are the cause of ichthyosis follicularis-atrichia-photophobia syndrome (IFAPS) [MIM:308205]. A syndrome characterized by a peculiar triad of follicular ichthyosis, total or subtotal atrichia, and photophobia of varying degree. Histopathologically, the epidermal granular layer is generally well-preserved or thickened at the infundibulum. Hair follicles are poorly developed and tend to be surrounded by an inflammatory infiltrate. A subgroup of patients is described with lamellar rather than follicular ichthyosis. Non-consistent features may include growth and psychomotor retardation, aganglionic megacolon, seizures and nail dystrophy.
    Defects in MBTPS2 are a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX) [MIM:308800]. A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration.
  • Sequence similarities

    Belongs to the peptidase M50A family.
  • Cellular localization

    Membrane. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRESEK antibody
    • Endopeptidase S2P antibody
    • IFAP antibody
    • KFSDX antibody
    • MBTP2_HUMAN antibody
    • MBTPS2 antibody
    • Membrane bound transcription factor site 2 protease antibody
    • membrane-bound transcription factor peptidase, site 2 antibody
    • membrane-bound transcription factor protease, site 2 antibody
    • Membrane-bound transcription factor site-2 protease antibody
    • S2P antibody
    • S2P endopeptidase antibody
    • Site 2 protease antibody
    • SREBPs intramembrane protease antibody
    • Sterol regulatory element-binding proteins intramembrane protease antibody
    see all


  • All lanes : Anti-S2P antibody (ab140594) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 26 kDa (possible non-specific binding), 40 kDa (possible non-specific binding), 62 kDa (possible glycosylated form)

    Exposure time: 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140594 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.


ab140594 has not yet been referenced specifically in any publications.

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