Overview

  • Product name

  • Description

    Rabbit polyclonal to S2P
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human S2P (internal sequence).
    Database link: O43462

  • Positive control

    • A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.

Target

  • Function

    Intramembrane proteolysis of sterol-regulatory element-binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
  • Involvement in disease

    Defects in MBTPS2 are the cause of ichthyosis follicularis-atrichia-photophobia syndrome (IFAPS) [MIM:308205]. A syndrome characterized by a peculiar triad of follicular ichthyosis, total or subtotal atrichia, and photophobia of varying degree. Histopathologically, the epidermal granular layer is generally well-preserved or thickened at the infundibulum. Hair follicles are poorly developed and tend to be surrounded by an inflammatory infiltrate. A subgroup of patients is described with lamellar rather than follicular ichthyosis. Non-consistent features may include growth and psychomotor retardation, aganglionic megacolon, seizures and nail dystrophy.
    Defects in MBTPS2 are a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX) [MIM:308800]. A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration.
  • Sequence similarities

    Belongs to the peptidase M50A family.
  • Cellular localization

    Membrane. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRESEK antibody
    • Endopeptidase S2P antibody
    • IFAP antibody
    • KFSDX antibody
    • MBTP2_HUMAN antibody
    • MBTPS2 antibody
    • Membrane bound transcription factor site 2 protease antibody
    • membrane-bound transcription factor peptidase, site 2 antibody
    • membrane-bound transcription factor protease, site 2 antibody
    • Membrane-bound transcription factor site-2 protease antibody
    • S2P antibody
    • S2P endopeptidase antibody
    • Site 2 protease antibody
    • SREBPs intramembrane protease antibody
    • Sterol regulatory element-binding proteins intramembrane protease antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling S2P with ab196797 at a 1/100 dilution. Right image treated with sythesized peptide.

References

ab196797 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 19 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (PC-3 prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
40 µg
Specification
PC-3 prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Nov 17 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Mar 28 2017

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