Key features and details
- Rabbit polyclonal to S31
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-S31 antibody
See all S31 primary antibodies
DescriptionRabbit polyclonal to S31
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig, Chimpanzee
- WB: HeLa whole cell lysate. IHC-P: Human testis, kidney and lung cancer tissues. ICC/IF: HeLa and PC-3 cells.
Previously labelled as 5HT1E Receptor.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 42 kDa.|
RelevanceThis is one of the several different receptors for 5-HT, a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. The 5-HT1E receptor is expressed in human cortex, caudate, putamen, and amygdala, as well as in dorsal root ganglia and in coronary artery. ESTs have been isolated from human brain cancer libraries.
Cellular localizationCell Membrane, Multi-pass membrane protein.
- 5 HT1E antibody
- 5 hydroxytryptamine (serotonin) receptor 1E antibody
- 5 hydroxytryptamine (serotonin) receptor 1E G protein coupled antibody
Anti-S31 antibody (ab236651) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 42 kDa
Paraffin-embedded human testis tissue stained for S31 using ab236651 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for S31 using ab236651 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for S31 (green) using ab236651 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
PC-3 (human prostate adenocarcinoma cell line) cells stained for S31 (green) using ab236651 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human lung cancer tissue stained for S31 using ab236651 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236651 has not yet been referenced specifically in any publications.