Anti-S6K1 antibody (ab9366)
Key features and details
- Rabbit polyclonal to S6K1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-S6K1 antibody
See all S6K1 primary antibodies -
Description
Rabbit polyclonal to S6K1 -
Host species
Rabbit -
Specificity
Reacts strongly with 70 kd of p70 S6K, but only weakly with the 80 kDa S6K. -
Tested Applications & Species
Application Species WB Mouse -
Immunogen
Synthetic peptide corresponding to Human S6K1 (C terminal) conjugated to keyhole limpet haemocyanin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.00
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography with S6K C-terminal peptide on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
WB |
Mouse
|
Application | Abreviews | Notes |
---|---|---|
WB | (8) |
Use at an assay dependent concentration. Predicted molecular weight: 59 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 59 kDa. |
Target
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Function
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. -
Post-translational
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. -
Cellular localization
Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 72508 Mouse
- SwissProt: Q8BSK8 Mouse
- Unigene: 394280 Mouse
- Unigene: 446624 Mouse
- Unigene: 479484 Mouse
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Alternative names
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
Datasheets and documents
References (25)
ab9366 has been referenced in 25 publications.
- Zha A et al. The Evaluation of the Antioxidant and Intestinal Protective Effects of Baicalin-Copper in Deoxynivalenol-Challenged Piglets. Oxid Med Cell Longev 2020:5363546 (2020). PubMed: 32064026
- Wang X et al. TFEB-NF-?B inflammatory signaling axis: a novel therapeutic pathway of Dihydrotanshinone I in doxorubicin-induced cardiotoxicity. J Exp Clin Cancer Res 39:93 (2020). PubMed: 32448281
- Lai WS & Ding YL GNG7 silencing promotes the proliferation and differentiation of placental cytotrophoblasts in preeclampsia rats through activation of the mTOR signaling pathway. Int J Mol Med 43:1939-1950 (2019). PubMed: 30864685
- Yang Y et al. Skullcapflavone I suppresses proliferation of human lung cancer cells via down-regulating microRNA-21. Exp Mol Pathol 110:104285 (2019). PubMed: 31301304
- Ma YF et al. Phosphorylation of AKT serine/threonine kinase and abundance of milk protein synthesis gene networks in mammary tissue in response to supply of methionine in periparturient Holstein cows. J Dairy Sci 102:4264-4274 (2019). PubMed: 30879806