Validated using a knockout cell line

Anti-S6K1 antibody [E175] (ab32359)


  • Product name
    Anti-S6K1 antibody [E175]
    See all S6K1 primary antibodies
  • Description
    Rabbit monoclonal [E175] to S6K1
  • Host species
  • Specificity
    ab32359 may detect both phosphorylated and non-phosphorylated forms of p70 S6 Kinase.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human S6K1. The exact sequence is proprietary.

  • Positive control
    • Jurkat cell lysate; human breast carcinoma
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab32359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/50.
IP 1/60.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • Domain
      The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
      The TOS (TOR signaling) motif is essential for activation by mTORC1.
    • Post-translational
      Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
    • Cellular localization
      Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 70 kDa ribosomal protein S6 kinase 1 antibody
      • KS6B1_HUMAN antibody
      • p70 alpha antibody
      • P70 beta 1 antibody
      • p70 ribosomal S6 kinase alpha antibody
      • p70 ribosomal S6 kinase beta 1 antibody
      • p70 S6 kinase alpha antibody
      • P70 S6 Kinase antibody
      • p70 S6 kinase, alpha 1 antibody
      • p70 S6 kinase, alpha 2 antibody
      • p70 S6K antibody
      • p70 S6K-alpha antibody
      • p70 S6KA antibody
      • p70(S6K) alpha antibody
      • p70(S6K)-alpha antibody
      • p70-alpha antibody
      • p70-S6K 1 antibody
      • p70-S6K antibody
      • P70S6K antibody
      • P70S6K1 antibody
      • p70S6Kb antibody
      • PS6K antibody
      • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
      • Ribosomal protein S6 kinase beta 1 antibody
      • Ribosomal protein S6 kinase beta-1 antibody
      • Ribosomal protein S6 kinase I antibody
      • RPS6KB1 antibody
      • S6K antibody
      • S6K-beta-1 antibody
      • S6K1 antibody
      • Serine/threonine kinase 14 alpha antibody
      • Serine/threonine-protein kinase 14A antibody
      • STK14A antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: S6K1 knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32359 observed at 68 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32359 was shown to recognize S6K1 when S6K1 knockout samples were used, along with additional cross-reactive bands. Wild-type and S6K1 knockout samples were subjected to SDS-PAGE. ab32359 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Paraffin-embedded human breast carcinoma
      ab32359 at 1/50 dilution
    • Anti-S6K1 antibody [E175] (ab32359) + 293T cell lysate

      Predicted band size: 59 kDa


    This product has been referenced in:
    • Liu F  et al. Increased T-helper 17 cell differentiation mediated by exosome-mediated microRNA-451 redistribution in gastric cancer infiltrated T cells. Cancer Sci 109:65-73 (2018). WB . Read more (PubMed: 29059496) »
    • Li H  et al. MEN1/Menin regulates milk protein synthesis through mTOR signaling in mammary epithelial cells. Sci Rep 7:5479 (2017). Read more (PubMed: 28710500) »

    See all 23 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting Abcam.

    I have gone ahead and refunded the cost of the order for ab32359 (see credit note information below) and also you should have received an email from FedEx with the shipping label for returning this antibody to ...

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    Thank you for contacting us. I can certainly compare the immunogens for our antibodies with a sequence for you. If you can provide the full length sequence you are interested in or the accession number, I will let you know which antibodies would be lik...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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    Thank you for contacting us.

    The exact epitope is proprietary, but the immunogen sequence is approximately 20 amino acids long and maps within amino acids 400-440 of p70 S6 Kinase of human origin.Thismeans, that both isoforms will be de...

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    Vielen Dank für Ihre Antwort.

    Die Referenznummer Ihrer Gutschrift ist 111111.

    Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, eine Gutsc...

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    Thank you for getting back to me.
    I can set up the order for the anti-smad2 (ps467) (ab53100) and anti-IKB (phospho s32 + s36) (ab12135) directly for you. Could you please just confirm that the delivery address I have for you is correct:

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    Thank you for your reply.

    I'm sending a free of charge vial of ab2571 on the order ***, which should ship out tonight and get to you tomorrow.

    Please keep me updated about the performance of this replacement antibody, and let me kno...

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    Thank you for your calls today and last week, and I am sorry for the trouble with our emails.

    As we discussed, we have ab2571 and ab19380 which are both phospho-specific S6K antibodies. Please let me know which you would like as a replacement...

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    Wenn wir eine Informationskampagne über einen Antikörper starten, versuchen wir natürlich immer, den Endverbraucher zu kontaktieren. Da aber viele Universitäten eine zentrale Einkaufsabteilung haben über die die Bestellungen abgewickelt werden, haben l...

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    Thank you for your telephone enquiry.

    I am sorry there have been some difficulties regarding the specificity of ab32359 S6K antibody [E175]

    As discussed on the telephone, I would be pleased to provide you with an alternative free of...

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