Product nameAnti-S6K1 antibody (HRP)
See all S6K1 primary antibodies
DescriptionRabbit polyclonal to S6K1 (HRP)
SpecificityReacts strongly with the 70 kDa of p70 S6K but only weakly to the 80 kDa S6K.
Tested applicationsSuitable for: WB, IP, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human, Pig
- Use Mouse brain extract.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesImmunoaffinity chromatography with S6K c-terminal peptide on agarose.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab9367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 3 µg/mg of lysate.|
FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1.
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
- Information by UniProt
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
One-step immunoblotting with cross-antibodies of the immunoprecipitated kinases
Immunoprecipitation with anti-p90 RSK1 (Lane A and C) Immunoprecipitation with anti-p70 S6K (Lane B and D) Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)
Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D)One-step immunoblotting with cross-antibodies of the immunoprecipitated kinases
Immunoprecipitation with anti-p90 RSK1 (Lane A and C)
Immunoprecipitation with anti-p70 S6K (Lane B and D)
Immunoblotting with anti-p70 S6K HRPconjugates (Lane A and B)
Immunoblotting with anti-RSK1 HRP conjugates (Lane C and D)
One-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).
Lane A: mouse Muscle
Lane B: mouse BrainOne-step immunoblotting of crude mouse brain and muscle samples with anti-S6K HRP conjugates (ECL system).
Lane A: mouse Muscle
Lane B: mouse Brain
ab9367 has not yet been referenced specifically in any publications.