Key features and details
- Mouse monoclonal [MM1061-9A34] to S6K1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-S6K1 antibody [MM1061-9A34]
See all S6K1 primary antibodies
DescriptionMouse monoclonal [MM1061-9A34] to S6K1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
Recombinant full length protein corresponding to Human S6K1 aa 1-502. AAA36411.
MAGVFDIDLD QPEDAGSEDE LEEGGQLNES MDHGGVGPYE LGMEHCEKFE ISETSVNRGP EKIRPECFEL LRVLGKGGYG KVFQVRKVTG ANTGKIFAMK VLKKAMIVRN AKDTAHTKAE RNILEEVKHP FIVDLIYAFQ TGGKLYLILE YLSGGELFMQ LEREGIFMED TACFYLAEIS MALGHLHQKG IIYRDLKPEN IMLNHQGHVK LTDFGLCKES IHDGTVTHTF CGTIEYMAPE ILMRSGHNRA VDWWSLGALM YDMLTGAPPF TGENRKKTID KILKCKLNLP PYLTQEARDL LKKLLKRNAA SRLGAGPGDA GEVQAHPFFR HINWEELLAR KVEPPFKPLL QSEEDVSQFD SKFTRQTPVD SPDDSTLSES ANQVFLGFTY VAPSVLESVK EKFSFEPKIR SPRRFIGSPR TPVSPVKFSP GDFWGRGASA STANPQTPVE YPMETSGIEQ MDVTMSGEAS APLPIRQPNS GPYKKQAFPM ISKRPEHLRM NL
Database link: P23443-2
- HeLa whole cell lysate (ab150035).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Purification notesThe IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
- Epigenetics and Nuclear Signaling
- DNA / RNA
- DNA Damage & Repair
- DNA Damage Response
- DNA Damage Recognition
Our Abpromise guarantee covers the use of ab211656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 56 kDa.|
FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1.
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
- Information by UniProt
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
ab211656 has been referenced in 1 publication.
- Lu J et al. MicroRNA-218-5p as a Potential Target for the Treatment of Human Osteoarthritis. Mol Ther 25:2676-2688 (2017). PubMed: 28919376