Overview

  • Product name

    Anti-S6K1 (phospho S424) antibody
    See all S6K1 primary antibodies
  • Description

    Rabbit polyclonal to S6K1 (phospho S424)
  • Host species

    Rabbit
  • Specificity

    ab131436 detects endogenous levels of S6K only when phosphorylated at serine 424.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Serine 424 (P-V-Sp-P-V) of Human S6K (UniProt P23443-2).

  • Positive control

    • MCF7 cell extract treated with EGF; HeLa cells; Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 70, 85 kDa (predicted molecular weight: 56, 59 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Domain

    The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
    The TOS (TOR signaling) motif is essential for activation by mTORC1.
  • Post-translational
    modifications

    Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
  • Cellular localization

    Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • KS6B1_HUMAN antibody
    • p70 alpha antibody
    • P70 beta 1 antibody
    • p70 ribosomal S6 kinase alpha antibody
    • p70 ribosomal S6 kinase beta 1 antibody
    • p70 S6 kinase alpha antibody
    • P70 S6 Kinase antibody
    • p70 S6 kinase, alpha 1 antibody
    • p70 S6 kinase, alpha 2 antibody
    • p70 S6K antibody
    • p70 S6K-alpha antibody
    • p70 S6KA antibody
    • p70(S6K) alpha antibody
    • p70(S6K)-alpha antibody
    • p70-alpha antibody
    • p70-S6K 1 antibody
    • p70-S6K antibody
    • P70S6K antibody
    • P70S6K1 antibody
    • p70S6Kb antibody
    • PS6K antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase beta 1 antibody
    • Ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • RPS6KB1 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • Serine/threonine kinase 14 alpha antibody
    • Serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

Images

  • All lanes : Anti-S6K1 (phospho S424) antibody (ab131436) at 1/500 dilution

    Lane 1 : MCF7 cell extract untreated
    Lane 2 : MCF7 cell extract treated with EGF

    Predicted band size: 56, 59 kDa
    Observed band size: 70,85 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling S6K (phospho S424) with ab131436 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling S6K (phospho S424) with ab131436 at 1/100 dilution.

References

This product has been referenced in:

  • Xu X  et al. Silencing the FOLR2 Gene Inhibits Cell Proliferation and Increases Apoptosis in the NCI-H1650 Non-Small Cell Lung Cancer Cell Line via Inhibition of AKT/Mammalian Target of Rapamycin (mTOR)/Ribosomal Protein S6 Kinase 1 (S6K1) Signaling. Med Sci Monit 24:8064-8073 (2018). Read more (PubMed: 30415267) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us in regards to the anti-S6K (phospho S424) antibody (ab131436).

I now have more information as to the bands presented on the datasheet of ab131436 and what size bands you can expect. The band presented at ˜70 kDa is p70 S6K, and the other band of ˜80 kDa is likely to bep85 S6K.The predicted band size of ˜59 kDa istaken from theSwissProt reference P23443 and is the predicted band size of p70 S6K without any post translational modifications. The differences between p70 S6K and p85 S6K can be reviewed from the following journal:



http://www.ncbi.nlm.nih.gov/pubmed/7803761.



From this information, it is likely that the band at ˜70 kDa you have been seeing is p70 S6K and the band at ˜52 kDa may either be a non-specific band, or may be the unmodified S6K protein.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

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