Anti-S6K1 (phospho T389 + T412) antibody (ab60948)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-S6K1 (phospho T389 + T412) antibody
See all S6K1 primary antibodies -
DescriptionRabbit polyclonal to S6K1 (phospho T389 + T412)
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Host speciesRabbit
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Specificityab60948 will detect both phopho-Thr389 of isoform alpha II and phospho-Thr412 of isoform alpha I.
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Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic phosphopeptide derived from isoform 2 of human p70 S6 Kinase beta-1 (P23443) around the phosphorylation site of threonine 389 (G-F-TP-Y-V).
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Positive control
- Human breast carcinoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab60948 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab60948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| ELISA | 1/1000. | |
| IHC-P | 1/50 - 1/100. | |
| WB | 1/500 - 1/1000. |
Target
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FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
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Tissue specificityWidely expressed.
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Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1. -
Post-translational
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism. -
Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
- Information by UniProt
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Database links
- Entrez Gene: 6198 Human
- Entrez Gene: 72508 Mouse
- Entrez Gene: 83840 Rat
- Omim: 608938 Human
- SwissProt: P23443 Human
- SwissProt: Q8BSK8 Mouse
- SwissProt: P67999 Rat
- Unigene: 463642 Human
see all -
Alternative names
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
see all
Images
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Western blot - Anti-S6K1 (phospho T389 + T412) antibody (ab60948)All lanes : Anti-S6K1 (phospho T389 + T412) antibody (ab60948)
Lane 1 : Jurkat cells treated with Insulin, 0.01U/ml
Lane 2 : Jurkat cells treated with Insulin, 0.01U/ml and phospho peptide used for blocking -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 (phospho T389 + T412) antibody (ab60948)ab60948 at 1/50 - 1/100 dilution staining P70 S6 Kinase beta in human breast carcinoma by Immunohistochemistry,
Paraffin embedded tissue, in the absence or presence of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-S6K1 (phospho T389 + T412) antibody (ab60948)ICC/IF image of ab60948 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60948, 1µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Cheng Z et al. Rab1A promotes proliferation and migration abilities via regulation of the HER2/AKT-independent mTOR/S6K1 pathway in colorectal cancer. Oncol Rep 41:2717-2728 (2019). Read more (PubMed: 30896866) »
- Qu Y et al. Phosphorylated 4EBP1 is associated with tumor progression and poor prognosis in Xp11.2 translocation renal cell carcinoma. Sci Rep 6:23594 (2016). IHC . Read more (PubMed: 27026382) »
