Key features and details
- Rabbit polyclonal to S6K1 (phospho T398)
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Product nameAnti-S6K1 (phospho T398) antibody
See all S6K1 primary antibodies
DescriptionRabbit polyclonal to S6K1 (phospho T398)
Specificityab228513 is specific for endogenous levels of the S6K1 protein phosphorylated at Threonine. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Drosophila melanogaster
Synthetic peptide corresponding to Drosophila melanogaster S6K1 (phospho T398).
Database link: Q94533
- WB: Drosophila S2 cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.01% BSA, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab228513 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Our Abpromise guarantee covers the use of ab228513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 55 kDa.|
FunctionActs to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
DomainThe autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
The TOS (TOR signaling) motif is essential for activation by mTORC1.
modificationsPhosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
Cellular localizationCytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
- Information by UniProt
- 70 kDa ribosomal protein S6 kinase 1 antibody
- KS6B1_HUMAN antibody
- p70 alpha antibody
All lanes : Anti-S6K1 (phospho T398) antibody (ab228513) at 1/1000 dilution
Lane 1 : Drosophila S2 cell lysate.
Lane 2 : Drosophila S2 cell lysate. with immunizing phosphopeptide
Predicted band size: 55 kDa
Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen (Lane 2), but not by the corresponding non-phosphopeptide (not shown)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228513 has not yet been referenced specifically in any publications.