Overview

  • Product name

    Anti-SA1 antibody - C-terminal
    See all SA1 primary antibodies
  • Description

    Rabbit polyclonal to SA1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human SA1 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q8WVM7

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab227736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 144 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similarities

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational
    modifications

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cohesin subunit SA 1 antibody
    • Cohesin subunit SA-1 antibody
    • Cohesin Subunit SA1 antibody
    • Nuclear protein stromal antigen 1 antibody
    • SA 1 antibody
    • SCC 3 homolog 1 antibody
    • Scc3 antibody
    • SCC3 homolog 1 antibody
    • STAG 1 antibody
    • stag1 antibody
    • STAG1_HUMAN antibody
    • Stromal antigen 1 antibody
    see all

Images

  • All lanes : Anti-SA1 antibody - C-terminal (ab227736) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SA1 shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 144 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-SA1 antibody - C-terminal (ab227736) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 144 kDa



    5% SDS-PAGE gel.

  • SA1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg of ab227736. Western blot was performed from the immunoprecipitate using ab227736.

    Lane 1: HeLa whole cell extract (Input). 

    Lane 2: Control IgG instaed of ab227736 in HeLa whole cell extract. 

    Lane 3: ab227736 IP in HeLa whole cell extract. 

References

ab227736 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227736.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up