• Product name

    Anti-SA1 antibody - ChIP Grade
    See all SA1 primary antibodies
  • Description

    Goat polyclonal to SA1 - ChIP Grade
  • Host species

  • Tested applications

    Suitable for: WB, ICC, ChIP, ChIP/Chipmore details
  • Species reactivity

    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human SA1 aa 1150-1200.



Our Abpromise guarantee covers the use of ab4457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
ICC 1/500 - 1/2000.
ChIP Use at an assay dependent concentration. PubMed: 18550811
ChIP/Chip Use at an assay dependent concentration. PubMed: 18550811


  • Function

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similarities

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cohesin subunit SA 1 antibody
    • Cohesin subunit SA-1 antibody
    • Cohesin Subunit SA1 antibody
    • Nuclear protein stromal antigen 1 antibody
    • SA 1 antibody
    • SCC 3 homolog 1 antibody
    • Scc3 antibody
    • SCC3 homolog 1 antibody
    • STAG 1 antibody
    • stag1 antibody
    • STAG1_HUMAN antibody
    • Stromal antigen 1 antibody
    see all


  • Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4457 at 1µg/ml.
  • Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4457 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

    Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4457 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.


This product has been referenced in:

  • Meisenberg C  et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). Read more (PubMed: 30554942) »
  • Steiner LA  et al. CTCF and CohesinSA-1 Mark Active Promoters and Boundaries of Repressive Chromatin Domains in Primary Human Erythroid Cells. PLoS One 11:e0155378 (2016). ChIP . Read more (PubMed: 27219007) »
See all 8 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


The epitope recognized by ab4457 maps to a region between residues 1150 and 1200 of human Stromal Antigen 1 using the numbering given in entry NP_005853.2 (GeneID 10274). I hope this helps, please contact us again if you have any additional questions.

Read More


Thank you very much for your patience. I'm afraid that I am unable to get this information from the originator of ab4455 and ab4457. I apologize for the inconvenience and if I can be of further assistance, please do let me know.

Read More


Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up