Product nameAnti-SA2 antibody
See all SA2 primary antibodies
DescriptionGoat polyclonal to SA2
SpecificityWe have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Xenopus laevis
Synthetic peptide corresponding to Human SA2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab4463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).|
|ICC||1/500 - 1/2000.|
FunctionComponent of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Sequence similaritiesBelongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain.
modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
- Information by UniProt
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml.
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4463 at 0.5
Detection: ECL with 15 second exposure.Nuclear Extract from HeLa cells (~5 x 10e5 cells).
ab4463 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
Goat polyclonal to SA2 antibody (ab4463) detecting Stromal antigen 2 in assynchronous HeLa cells, together with a Donkey anti-goat secondary antibody conjugated to Cy3 and DAPI as counterstain. Please refer to abreview for further protocol details.
This product has been referenced in:
- Fukuda T et al. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J 33:1243-55 (2014). Read more (PubMed: 24797475) »
- Ishiguro K et al. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing. EMBO Rep 12:267-75 (2011). WB ; Mouse . Read more (PubMed: 21274006) »