• Product name
  • Description
    Goat polyclonal to SA2
  • Host species
  • Specificity
    We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human, Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human SA2.



Our Abpromise guarantee covers the use of ab4463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC 1/500 - 1/2000.
ICC/IF 1/200.


  • Function
    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similarities
    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational
    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA517O1.1 antibody
    • Cohesin Subunit SA 2 antibody
    • Cohesin subunit SA-2 antibody
    • DKFZp686P168 antibody
    • DKFZp781H1753 antibody
    • FLJ25871 antibody
    • SA 2 antibody
    • SA-2 antibody
    • SA2 antibody
    • SCC3 homolog 2 antibody
    • SCC3B antibody
    • STAG 2 antibody
    • stag2 antibody
    • STAG2_HUMAN antibody
    • Stromal antigen 2 antibody
    see all


  • Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4463 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

    Nuclear Extract from HeLa cells (~5 x 10e5 cells).

    ab4463 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.
  • Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml.
  • Goat polyclonal to SA2 antibody (ab4463) detecting Stromal antigen 2 in assynchronous HeLa cells, together with a Donkey anti-goat secondary antibody conjugated to Cy3 and DAPI as counterstain. Please refer to abreview for further protocol details.

    See Abreview


This product has been referenced in:
  • Meisenberg C  et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). Read more (PubMed: 30554942) »
  • Fukuda T  et al. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J 33:1243-55 (2014). Read more (PubMed: 24797475) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 16 2007


Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

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