Anti-SA2 antibody (ab4463)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-SA2 antibody
See all SA2 primary antibodies -
DescriptionGoat polyclonal to SA2
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Host speciesGoat
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SpecificityWe have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
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Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human SA2.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab4463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). | |
| ICC | 1/500 - 1/2000. | |
| ICC/IF | 1/200. |
Target
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FunctionComponent of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
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Sequence similaritiesBelongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
Post-translational
modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
- Information by UniProt
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Database links
- Entrez Gene: 10735 Human
- Entrez Gene: 20843 Mouse
- Entrez Gene: 394319 Xenopus laevis
- Omim: 300826 Human
- SwissProt: Q8N3U4 Human
- SwissProt: O35638 Mouse
- SwissProt: Q9DGN0 Xenopus laevis
- Unigene: 496710 Human
see all -
Alternative names
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
see all
Images
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Western blot - Anti-SA2 antibody (ab4463)Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4463 at 0.5
µ g/ml.Detection: ECL with 15 second exposure.
Nuclear Extract from HeLa cells (~5 x 10e5 cells).
ab4463 at 0.5 µg/ml.
Detection: ECL with 15 second exposure. -
Immunocytochemistry - Anti-SA2 antibody (ab4463)Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-SA2 antibody (ab4463)This image is courtesy of an Abreview submitted by Dr Kirk McManusGoat polyclonal to SA2 antibody (ab4463) detecting Stromal antigen 2 in assynchronous HeLa cells, together with a Donkey anti-goat secondary antibody conjugated to Cy3 and DAPI as counterstain. Please refer to abreview for further protocol details.
References
This product has been referenced in:
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). Read more (PubMed: 30554942) »
- Fukuda T et al. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J 33:1243-55 (2014). Read more (PubMed: 24797475) »
