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    sa2-antibody-ab4463.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Anti-SA2 antibody (ab4463)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (7)

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Western blot - Anti-SA2 antibody (ab4463)
  • Immunocytochemistry - Anti-SA2 antibody (ab4463)

Key features and details

  • Goat polyclonal to SA2
  • Suitable for: WB, ICC
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SA2 antibody
    See all SA2 primary antibodies
  • Description

    Goat polyclonal to SA2
  • Host species

    Goat
  • Specificity

    We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human SA2.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab4463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC
1/500 - 1/2000.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC
1/500 - 1/2000.

Target

  • Function

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similarities

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational
    modifications

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Target information above from: UniProt accession Q8N3U4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10735 Human
    • Omim: 300826 Human
    • SwissProt: Q8N3U4 Human
    • Unigene: 496710 Human
    • Unigene: 624663 Human
    • Alternative names

      • bA517O1.1 antibody
      • Cohesin Subunit SA 2 antibody
      • Cohesin subunit SA-2 antibody
      • DKFZp686P168 antibody
      • DKFZp781H1753 antibody
      • FLJ25871 antibody
      • SA 2 antibody
      • SA-2 antibody
      • SA2 antibody
      • SCC3 homolog 2 antibody
      • SCC3B antibody
      • STAG 2 antibody
      • stag2 antibody
      • STAG2_HUMAN antibody
      • Stromal antigen 2 antibody
      see all

    Images

    • Western blot - Anti-SA2 antibody (ab4463)
      Western blot - Anti-SA2 antibody (ab4463)

      Nuclear Extract from HeLa cells (~5 x 105 cells).

      ab4463 at 0.5 µg/ml.

      Detection: ECL with 15 second exposure.

      Nuclear Extract from HeLa cells (~5 x 10e5 cells).

      ab4463 at 0.5 µg/ml.

      Detection: ECL with 15 second exposure.
    • Immunocytochemistry - Anti-SA2 antibody (ab4463)
      Immunocytochemistry - Anti-SA2 antibody (ab4463)
      Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4463 at 1µg/ml.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab4463? Please let us know so that we can cite the reference in this datasheet.

    ab4463 has been referenced in 7 publications.

    • Meisenberg C  et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
    • Fukuda T  et al. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J 33:1243-55 (2014). PubMed: 24797475
    • Ishiguro K  et al. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing. EMBO Rep 12:267-75 (2011). WB ; Mouse . PubMed: 21274006
    • Xiao T  et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . PubMed: 21444719
    • Tanno Y  et al. Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres. Genes Dev 24:2169-79 (2010). WB ; Human . PubMed: 20889715
    • Yao H  et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). WB ; Human . PubMed: 20966046
    • Sun Y  et al. Separase is recruited to mitotic chromosomes to dissolve sister chromatid cohesion in a DNA-dependent manner. Cell 137:123-32 (2009). WB ; Human . PubMed: 19345191

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SA2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Apr 16 2007

    Question

    I have a question related to the four types of goat anti-SCC3 antibodies that you have (Ab4455, Ab4457, Ab4463, and Ab4464). Do you know which one works best on immunostaining?

    Read More

    Abcam community

    Verified customer

    Asked on May 05 2004

    Answer

    Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on May 06 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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