Recombinant
RabMAb

Anti-SA2 antibody [EPR10993] - N-terminal (ab171970)

Overview

  • Product name
    Anti-SA2 antibody [EPR10993] - N-terminal
    See all SA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10993] to SA2 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SA2 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8N3U4

  • Positive control
    • MCF7 and Jurkat cells. Jurkat, K-562, MCF7 and HeLa cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 141 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
    • Sequence similarities
      Belongs to the SCC3 family.
      Contains 1 SCD (stromalin conservative) domain.
    • Post-translational
      modifications
      Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
    • Cellular localization
      Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA517O1.1 antibody
      • Cohesin Subunit SA 2 antibody
      • Cohesin subunit SA-2 antibody
      • DKFZp686P168 antibody
      • DKFZp781H1753 antibody
      • FLJ25871 antibody
      • SA 2 antibody
      • SA-2 antibody
      • SA2 antibody
      • SCC3 homolog 2 antibody
      • SCC3B antibody
      • STAG 2 antibody
      • stag2 antibody
      • STAG2_HUMAN antibody
      • Stromal antigen 2 antibody
      see all

    Images

    • All lanes : Anti-SA2 antibody [EPR10993] - N-terminal (ab171970) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K-562 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 141 kDa

    • Immunofluorescence analysis of MCF7 cells, labeling SA2 using ab171970 at a 1/100 dilution.

    • Flow cytometric analysis of permeabilized Jurkat cells, labeling SA2 using ab171970 at a 1/10 dilution (red) or a rabbit IgG as negative control (green).

    References

    ab171970 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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