Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10993] to SA2 - N-terminal
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-SA2 antibody [EPR10993] - N-terminal
See all SA2 primary antibodies
DescriptionRabbit monoclonal [EPR10993] to SA2 - N-terminal
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SA2 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q8N3U4
- MCF7 and Jurkat cells. Jurkat, K-562, MCF7 and HeLa cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 141 kDa.|
FunctionComponent of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Sequence similaritiesBelongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain.
modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
Cellular localizationNucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
- Information by UniProt
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
All lanes : Anti-SA2 antibody [EPR10993] - N-terminal (ab171970) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K-562 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Immunofluorescence analysis of MCF7 cells, labeling SA2 using ab171970 at a 1/100 dilution.
Flow cytometric analysis of permeabilized Jurkat cells, labeling SA2 using ab171970 at a 1/10 dilution (red) or a rabbit IgG as negative control (green).
ab171970 has not yet been referenced specifically in any publications.