Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HeLa cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Detection
Detection method
ECL
Negative control
SA2 KO HeLa cell line
Exposure
40 second(s)
Bands
Specific: 141 kDa Non-specific: 100 kDa
Positive control
Wt HeLa cell line, SA2 KO-OE-isoform-1 HeLa cell line, SA2 KO-OE-isoform-2 HeLa cell line
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
ziying xie
Verified customer
Submitted Nov 17 2023