Recombinant
RabMAb

Anti-SA2 antibody [EPR17865] - C-terminal (HRP) (ab209477)

Overview

  • Product name
    Anti-SA2 antibody [EPR17865] - C-terminal (HRP)
    See all SA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR17865] to SA2 - C-terminal (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human SA2 aa 1000 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N3U4

  • Positive control
    • WB: K562 cell lysate. IHC-P: normal human tonsil tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab209477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 141 kDa (predicted molecular weight: 141 kDa).

Target

  • Function
    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similarities
    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational
    modifications
    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localization
    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA517O1.1 antibody
    • Cohesin Subunit SA 2 antibody
    • Cohesin subunit SA-2 antibody
    • DKFZp686P168 antibody
    • DKFZp781H1753 antibody
    • FLJ25871 antibody
    • SA 2 antibody
    • SA-2 antibody
    • SA2 antibody
    • SCC3 homolog 2 antibody
    • SCC3B antibody
    • STAG 2 antibody
    • stag2 antibody
    • STAG2_HUMAN antibody
    • Stromal antigen 2 antibody
    see all

Images

  • IHC image of SA2 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab209477, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-SA2 antibody [EPR17865] - C-terminal (HRP) (ab209477) at 1/5000 dilution + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 141 kDa
    Observed band size: 141 kDa


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209477 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209477 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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