Anti-SA2 antibody - N-terminal (ab229681)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SA2 antibody - N-terminal
See all SA2 primary antibodies -
Description
Rabbit polyclonal to SA2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow
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Immunogen
Recombinant fragment within Human SA2 (N terminal). The exact sequence is proprietary.
Database link: Q8N3U4 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. IP: HEK-293T whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- A431 whole cell lysate (ab30132)
- NIH 3T3 whole cell lysate (ab7179)
- C2C12 whole cell lysate (ab7182)
- RAW 264.7 whole cell lysate (ab7187)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- 293T whole cell lysate (ab95494)
Applications
Our Abpromise guarantee covers the use of ab229681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 141 kDa. | |
| IP | 1/100 - 1/500. |
Target
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Function
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
Sequence similarities
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
Post-translational
modificationsPhosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. - Information by UniProt
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Database links
- Entrez Gene: 10735 Human
- Entrez Gene: 20843 Mouse
- Entrez Gene: 313304 Rat
- Omim: 300826 Human
- SwissProt: Q8N3U4 Human
- SwissProt: O35638 Mouse
- Unigene: 496710 Human
- Unigene: 624663 Human
see all -
Alternative names
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
see all
Images
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Western blot - Anti-SA2 antibody - N-terminal (ab229681)All lanes : Anti-SA2 antibody - N-terminal (ab229681) at 1/2000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SA2 shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 141 kDa5% SDS-PAGE gel.
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Western blot - Anti-SA2 antibody - N-terminal (ab229681)All lanes : Anti-SA2 antibody - N-terminal (ab229681) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 141 kDa5% SDS-PAGE gel.
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Western blot - Anti-SA2 antibody - N-terminal (ab229681)All lanes : Anti-SA2 antibody - N-terminal (ab229681) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 141 kDa5% SDS-PAGE gel.
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Immunoprecipitation - Anti-SA2 antibody - N-terminal (ab229681)SA2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229681. Western blot was performed from the immunoprecipitate using ab229681. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG instead of ab227524 in HEK-293T whole cell extract.
Lane 2: ab229681 IP in HEK-293T whole cell extract.
References
ab229681 has not yet been referenced specifically in any publications.
