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    sa2-antibody-n-terminal-ab229681.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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Anti-SA2 antibody - N-terminal (ab229681)

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Western blot - Anti-SA2 antibody - N-terminal (ab229681)
  • Western blot - Anti-SA2 antibody - N-terminal (ab229681)
  • Western blot - Anti-SA2 antibody - N-terminal (ab229681)
  • Immunoprecipitation - Anti-SA2 antibody - N-terminal (ab229681)

Key features and details

  • Rabbit polyclonal to SA2 - N-terminal
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SA2 antibody - N-terminal
    See all SA2 primary antibodies
  • Description

    Rabbit polyclonal to SA2 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Recombinant fragment within Human SA2 (N terminal). The exact sequence is proprietary.
    Database link: Q8N3U4

  • Positive control

    • WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7 and C2C12 whole cell extracts. IP: HEK-293T whole cell extract.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab30132)
    • NIH/3T3 whole cell lysate (ab7179)
    • C2C12 whole cell lysate (ab7182)
    • RAW 264.7 whole cell lysate (ab7187)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab229681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 141 kDa.
IP
1/100 - 1/500.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 141 kDa.
IP
1/100 - 1/500.

Target

  • Function

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similarities

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational
    modifications

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Target information above from: UniProt accession Q8N3U4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10735 Human
    • Entrez Gene: 20843 Mouse
    • Entrez Gene: 313304 Rat
    • Omim: 300826 Human
    • SwissProt: Q8N3U4 Human
    • SwissProt: O35638 Mouse
    • Unigene: 496710 Human
    • Unigene: 624663 Human
    • Unigene: 290422 Mouse
    see all
  • Alternative names

    • bA517O1.1 antibody
    • Cohesin Subunit SA 2 antibody
    • Cohesin subunit SA-2 antibody
    • DKFZp686P168 antibody
    • DKFZp781H1753 antibody
    • FLJ25871 antibody
    • SA 2 antibody
    • SA-2 antibody
    • SA2 antibody
    • SCC3 homolog 2 antibody
    • SCC3B antibody
    • STAG 2 antibody
    • stag2 antibody
    • STAG2_HUMAN antibody
    • Stromal antigen 2 antibody
    see all

Images

  • Western blot - Anti-SA2 antibody - N-terminal (ab229681)
    Western blot - Anti-SA2 antibody - N-terminal (ab229681)
    All lanes : Anti-SA2 antibody - N-terminal (ab229681) at 1/2000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with SA2 shRNA, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 141 kDa



    5% SDS-PAGE gel.

  • Western blot - Anti-SA2 antibody - N-terminal (ab229681)
    Western blot - Anti-SA2 antibody - N-terminal (ab229681)
    All lanes : Anti-SA2 antibody - N-terminal (ab229681) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 141 kDa



    5% SDS-PAGE gel.

  • Western blot - Anti-SA2 antibody - N-terminal (ab229681)
    Western blot - Anti-SA2 antibody - N-terminal (ab229681)
    All lanes : Anti-SA2 antibody - N-terminal (ab229681) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 141 kDa



    5% SDS-PAGE gel.

  • Immunoprecipitation - Anti-SA2 antibody - N-terminal (ab229681)
    Immunoprecipitation - Anti-SA2 antibody - N-terminal (ab229681)

    SA2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab229681. Western blot was performed from the immunoprecipitate using ab229681. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG instead of ab227524 in HEK-293T whole cell extract. 
    Lane 2: ab229681 IP in HEK-293T whole cell extract. 

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab229681? Please let us know so that we can cite the reference in this datasheet.

ab229681 has not yet been referenced specifically in any publications.

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