Recombinant
RabMAb

Recombinant Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030)

Overview

  • Product name

    Anti-SAA1 + SAA2 antibody [EPR19235]
    See all SAA1 + SAA2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19235] to SAA1 + SAA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein within Mouse SAA1 + SAA2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P05366

  • Positive control

    • WB: Recombinant mouse SAA1 protein (aa20-122); GST and His-tagged recombinant mouse SAA2 protein (aa20-122); Mouse plasma and serum. IHC-P: Mouse liver tissue. IHC-Fr: Mouse liver tissue. IP: Mouse serum.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19235
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab199030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.

Antigen retrieval: Heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20).

IP 1/40.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).

Target

  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • PIG4 antibody
    • SAA antibody
    • SAA1 antibody
    • SAA2 antibody
    • Serum amyloid A 2 protein antibody
    • Serum amyloid A protein antibody
    • Serum amyloid A1 antibody
    • Serum amyloid A2 antibody
    • TP53I4 antibody
    • Tumor protein p53 inducible protein 4 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse liver is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SAA1 + SAA2 with ab199030 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on mouse kidney.

    Negative control: mouse kidney (PMID:19747761; PMID: 15623807).

    Counter stained with Hematoxylin.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling SAA1 + SAA2 with ab199030 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/100 dilution (green). Cytoplasmic staining on mouse liver is observed. 

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • All lanes : Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/10000 dilution

    Lane 1 : Recombinant mouse SAA1 protein (aa20-122) at 0.01 µg
    Lane 2 : GST and His-tagged recombinant mouse SAA2 protein (aa20-122) at 0.005 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/2000 dilution + GST and His-tagged recombinant mouse SAA3 protein (aa1-122) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-SAA1 + SAA2 antibody [EPR19235] (ab199030) at 1/1000 dilution

    Lane 1 : Mouse plasma
    Lane 2 : Mouse serum

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 13 kDa
    Observed band size: 13 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 30 seconds.

  • SAA1 + SAA2 was immunoprecipitated from 1 mg of mouse serum with ab199030 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199030 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse serum 10 μg (Input).

    Lane 2: ab199030 IP in mouse serum.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199030 in mouse serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

References

This product has been referenced in:

  • Meng X  et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). Read more (PubMed: 30773478) »
See 1 Publication for this product

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