Recombinant Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19550] to SAA1 + SAA2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free
See all SAA1 + SAA2 primary antibodies -
Description
Rabbit monoclonal [EPR19550] to SAA1 + SAA2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251476 is the carrier-free version of ab207445.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19550 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Cellular localization
Secreted -
Database links
- Entrez Gene: 6288 Human
- Entrez Gene: 6289 Human
- Omim: 104750 Human
- Omim: 104751 Human
- SwissProt: P0DJI8 Human
- SwissProt: P0DJI9 Human
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Alternative names
- PIG4 antibody
- SAA antibody
- SAA1 antibody
see all
Images
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All lanes : Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/1000 dilution
Lane 1 : Human SAA1 active recombinant protein
Lane 2 : Human SAA2 fragment recombinant protein
Lane 3 : Human SAA4 fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 13,35 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab207445, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human SAA2 and SAA4 fragment recombinant protein contain aa19-122 and aa19-130 with His-Tag® and GST-tag.
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Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/5000 dilution + Human serum at 20 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 3 minutesThis data was developed using ab207445, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Expressed by the liver; secreted in plasma and serum (PMID: 3183061; 16039662).
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Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/1000 dilution + Human liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 3 minutesThis data was developed using ab207445, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Expressed by the liver; secreted in plasma and serum (PMID: 3183061; 16039662).
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This data was developed using ab207445, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab207445, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251476 has not yet been referenced specifically in any publications.