Anti-SACM1L/Sac1 antibody (ab230642)
Key features and details
- Rabbit polyclonal to SACM1L/Sac1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SACM1L/Sac1 antibody
See all SACM1L/Sac1 primary antibodies -
Description
Rabbit polyclonal to SACM1L/Sac1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SACM1L/Sac1 aa 400-500 conjugated to keyhole limpet haemocyanin.
Database link: Q9NTJ5 -
Positive control
- WB: Jurkat whole cell lysate (ab7899). IHC-P: Human kidney and colon tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 67 kDa.
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IHC-P |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 67 kDa. |
IHC-P
1/100. |
Target
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Function
Phosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2. -
Tissue specificity
Detected in heart, brain, lung, liver, kidney, pancreas and testis. -
Sequence similarities
Contains 1 SAC domain. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 530577 Cow
- Entrez Gene: 22908 Human
- Entrez Gene: 83493 Mouse
- Entrez Gene: 100172954 Orangutan
- Entrez Gene: 116482 Rat
- Omim: 606569 Human
- SwissProt: A6QL88 Cow
- SwissProt: Q9NTJ5 Human
see all -
Alternative names
- Phosphatidylinositide phosphatase SAC1 antibody
- SAC1 antibody
- SAC1 suppressor of actin mutations 1-like (yeast) antibody
see all
Images
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Anti-CSRP2BP antibody (ab230641) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 67 kDa -
Formalin-fixed, paraffin-embedded human kidney tissue stained for SACM1L/Sac1 with ab230642 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for SACM1L/Sac1 with ab230642 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab230642 has been referenced in 1 publication.
- Pathmasiri KC et al. Mass spectrometry imaging and LC/MS reveal decreased cerebellar phosphoinositides in Niemann-Pick type C1-null mice. J Lipid Res 61:1004-1013 (2020). PubMed: 32371566