Anti-Sacsin antibody (ab242224)
Key features and details
- Rabbit polyclonal to Sacsin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sacsin antibody
See all Sacsin primary antibodies -
Description
Rabbit polyclonal to Sacsin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Sacsin aa 4529-4579. The exact sequence is proprietary.
Database link: Q9NZJ4 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whle cell lysates. IP: Sacsin IP in Jurkat whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Sacsin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 521 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins. -
Tissue specificity
Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas. -
Involvement in disease
Spastic ataxia Charlevoix-Saguenay type -
Sequence similarities
Contains 1 HEPN domain.
Contains 1 J domain.
Contains 1 ubiquitin-like domain. -
Domain
The ubiquitin-like domain mediates interaction with the proteasome.
The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity. -
Cellular localization
Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60. - Information by UniProt
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Database links
- Entrez Gene: 26278 Human
- Omim: 604490 Human
- SwissProt: Q9NZJ4 Human
- Unigene: 159492 Human
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Alternative names
- DnaJ homolog subfamily C member 29 antibody
- DNAJC29 antibody
- SACS antibody
see all
Images
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Western blot - Anti-Sacsin antibody (ab242224)All lanes : Anti-Sacsin antibody (ab242224) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 521 kDa
Exposure time: 3 minutesLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-Sacsin antibody (ab242224)Sacsin was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242224 at 6 µg/reaction.
Lysates prepared in NETN buffer.
All lanes : Anti-Sacsin antibody (ab242224) at 1 µg/ml
Lane 1 : ab242224 IP in Jurkat whole cel lysate
Lane 2 : Control IgG IP in HEK-203T whole cell lysate.
Developed using the ECL technique.
Exposure time: 30 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab242224 has not yet been referenced specifically in any publications.
