Anti-Sacsin antibody (ab242224)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Sacsin antibody
See all Sacsin primary antibodies -
Description
Rabbit polyclonal to Sacsin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Sacsin aa 4529-4579. The exact sequence is proprietary.
Database link: Q9NZJ4 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whle cell lysates. IP: Sacsin IP in Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Sacsin immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab242224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 521 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins. -
Tissue specificity
Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas. -
Involvement in disease
Spastic ataxia Charlevoix-Saguenay type -
Sequence similarities
Contains 1 HEPN domain.
Contains 1 J domain.
Contains 1 ubiquitin-like domain. -
Domain
The ubiquitin-like domain mediates interaction with the proteasome.
The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity. -
Cellular localization
Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60. - Information by UniProt
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Database links
- Entrez Gene: 26278 Human
- Omim: 604490 Human
- SwissProt: Q9NZJ4 Human
- Unigene: 159492 Human
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Alternative names
- DnaJ homolog subfamily C member 29 antibody
- DNAJC29 antibody
- SACS antibody
see all
Images
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Western blot - Anti-Sacsin antibody (ab242224)All lanes : Anti-Sacsin antibody (ab242224) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 521 kDa
Exposure time: 3 minutesLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-Sacsin antibody (ab242224)Sacsin was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242224 at 6 µg/reaction.
Lysates prepared in NETN buffer.
All lanes : Anti-Sacsin antibody (ab242224) at 1 µg/ml
Lane 1 : ab242224 IP in Jurkat whole cel lysate
Lane 2 : Control IgG IP in HEK-203T whole cell lysate.
Developed using the ECL technique.
Exposure time: 30 seconds
Datasheets and documents
References
ab242224 has not yet been referenced specifically in any publications.
