Recombinant Anti-Sacsin antibody [EPR11864] (ab181190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11864] to Sacsin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Sacsin antibody [EPR11864]
See all Sacsin primary antibodies -
Description
Rabbit monoclonal [EPR11864] to Sacsin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain lysate. Human glioma lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11864 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 521 kDa.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 521 kDa. |
Target
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Function
Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins. -
Tissue specificity
Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas. -
Involvement in disease
Spastic ataxia Charlevoix-Saguenay type -
Sequence similarities
Contains 1 HEPN domain.
Contains 1 J domain.
Contains 1 ubiquitin-like domain. -
Domain
The ubiquitin-like domain mediates interaction with the proteasome.
The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity. -
Cellular localization
Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60. - Information by UniProt
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Database links
- Entrez Gene: 26278 Human
- Omim: 604490 Human
- SwissProt: Q9NZJ4 Human
- Unigene: 159492 Human
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Alternative names
- DnaJ homolog subfamily C member 29 antibody
- DNAJC29 antibody
- SACS antibody
see all
Images
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Anti-Sacsin antibody [EPR11864] (ab181190) at 1/5000 dilution + Human glioma tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 521 kDa
Additional bands at: 437 kDa (possible isoform) -
Anti-Sacsin antibody [EPR11864] (ab181190) at 1/2000 dilution + Human fetal brain at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 521 kDa
Additional bands at: 437 kDa (possible isoform)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab181190 has been referenced in 3 publications.
- Romano LEL et al. Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization. Cell Rep 41:111580 (2022). PubMed: 36323248
- Longo F et al. Assessment of Sacsin Turnover in Patients With ARSACS: Implications for Molecular Diagnosis and Pathogenesis. Neurology 97:e2315-e2327 (2021). PubMed: 34649874
- Larivière R et al. Sacs R272C missense homozygous mice develop an ataxia phenotype. Mol Brain 12:19 (2019). PubMed: 30866998